Department of Plant Protection, Faculty of Agriculture, University of Ordu, Ordu, 52200, Türkiye.
Mol Biol Rep. 2024 Aug 19;51(1):918. doi: 10.1007/s11033-024-09843-5.
Root-lesion nematodes (RLN) are the most economically important pathogenic nematodes attacking maize. Significant economic losses due to lesion nematodes have been reported in maize producing countries in the world.
This study was conducted to determine the distribution and identity of root-lesion nematodes (Pratylenchus spp.) (Tylenchida: Hoplolaimidae) in maize (Zea mays L.) (Poales: Poaceae) fields of the Black Sea region of Türkiye. For this purpose, 39 locations were surveyed and soil samples were taken from 17 regional provinces. Nematodes were extracted using the modified Baerman funnel technique. The species were identified based on sequences of the Internal Transcribed Spacer (ITS) region of ribosomal DNA, as well as morphological characters and morphometrics. In addition, species identifications were confirmed using species-specific primers in the D3 expansion region of 26 S rDNA. At the end of the study, 51.3% of the maize production areas sampled in the region were infected with root-lesion nematode species. Pratylenchus agilis, P. mediterraneus, P. neglectus, P. penetrans, P. thornei, and P. vulnus were identified, and were present in 25%, 5%, 25%, 10%, 15%, and 20% of samples, respectively. To our knowledge, this is the first report of P. agilis in Türkiye.
The present study concluded that the molecular analysis of Pratylenchus sequences based on the ITS and D3 region of ribosomal RNA genes allowed the identification of six root lesion nematode species. This study is of great importance in terms of adding additional species to the root-lesion nematode fauna in Turkey and will provide data for future research on the management of these nematodes.
根结线虫是危害玉米的最重要的致病性线虫。世界上玉米生产国因根结线虫造成了重大经济损失。
本研究旨在确定土耳其黑海地区玉米（Zea mays L.）（禾本科：禾本科）田中根结线虫（Pratylenchus spp.）（侧尾目：滑刃科）的分布和种类。为此，调查了 39 个地点，并从 17 个地区采集了土壤样本。使用改良的 Baerman 漏斗技术提取线虫。根据核糖体 DNA 的内转录间隔区（ITS）序列、形态特征和形态计量学，对物种进行鉴定。此外，还使用 26S rDNA 的 D3 扩展区的种特异性引物确认了物种鉴定。研究结束时，该地区采样的玉米生产区中有 51.3%感染了根结线虫。鉴定出活跃短体线虫、地中海短体线虫、疏忽短体线虫、穿透短体线虫、索氏短体线虫和脆弱短体线虫，分别在 25%、5%、25%、10%、15%和 20%的样本中出现。据我们所知，这是在土耳其首次报道活跃短体线虫。
本研究基于核糖体 RNA 基因的 ITS 和 D3 区的 Pratylenchus 序列的分子分析，鉴定出了 6 种根结线虫。本研究对于在土耳其增加根结线虫区系的额外物种具有重要意义，并将为未来管理这些线虫的研究提供数据。
