网络药理学研究异荭草苷诱导胃癌细胞凋亡和抑制侵袭迁移的作用机制。

Apoptosis induction and inhibition of invasion and migration in gastric cancer cells by Isoorientin studied using network pharmacology.

机构信息

Joint Laboratory for Research on Active Components and Pharmacological Mechanism of Tibetan Materia Medica of Tibetan Medical Research Center of Tibet, School of Medicine, Xizang Minzu University, Xianyang, 712082, China.

Engineering Research Center of Tibetan Medicine Detection Technology, Ministry of Education, Xizang Minzu University, Xianyang, 712082, China.

出版信息

BMC Complement Med Ther. 2024 Aug 19;24(1):309. doi: 10.1186/s12906-024-04605-z.

DOI:10.1186/s12906-024-04605-z

PMID:39160561

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11334567/

Abstract

BACKGROUND

To investigate the effects of Isoorientin on the apoptosis, proliferation, invasion, and migration of human gastric cancer cells (HGC27 cells).

METHODS

We used network pharmacology to predict the targets of drugs and diseases. The CCK-8 assay was used to determine the effects of Isoorientin on the proliferation of HGC27 cells. Flow cytometry was employed to analyze the effects of Isoorientin on cell apoptosis and cell cycle distribution of HGC27 cells. Scratch test and transwell chamber test were conducted to assess the effects of Isoorientin on invasion and migration, respectively. Additionally, qPCR and western blot were performed to examine the impact of Isoorientin on apoptosis-related genes and protein expression, respectively.

RESULTS

The Isoorientin significantly inhibited the proliferation, migration, and invasion of HGC27 cells compared to the control group. Furthermore, Isoorientin induced apoptosis in HGC27 cells by upregulating the relative expression of Bax and caspase-3 while downregulating the relative expression of p-PI3K, p-AKT, and Bcl-2 proteins.

CONCLUSION

The Isoorientin exhibits inhibitory effects on the proliferation, invasion, and migration of HGC27 cells, and induces apoptosis in gastric cancer cells.

摘要

背景

研究染料木素异黄酮对人胃癌细胞（HGC27 细胞）凋亡、增殖、侵袭和迁移的影响。

方法

我们利用网络药理学预测药物和疾病的靶点。CCK-8 法测定染料木素异黄酮对 HGC27 细胞增殖的影响。流式细胞术分析染料木素异黄酮对 HGC27 细胞凋亡和细胞周期分布的影响。划痕试验和 Transwell 室试验分别评估染料木素异黄酮对侵袭和迁移的影响。此外，qPCR 和 Western blot 分别检测染料木素异黄酮对凋亡相关基因和蛋白表达的影响。