Ofeniforo Bankole Emmanuel, Ogunro Olalekan Bukunmi, Dike Charles Ebere, Agada Eleojo Samuel, Akinwunmi Kemi Feyisayo
Department of Chemical Sciences, Faculty of Natural and Applied Science, Oduduwa University Ipetumodu, Ile-Ife, Nigeria.
Department of Biochemistry, Faculty of Life Science, University of Ilorin, Ilorin, Nigeria.
Vector Borne Zoonotic Dis. 2025 Jan;25(1):26-33. doi: 10.1089/vbz.2024.0039. Epub 2024 Aug 20.
Malaria airs a life-threatening risk in Tropical African countries, stemming from infection by species. This region is richly endowed by nature with a wealth of diverse and largely unexplored plants that hold the potential for managing this protozoan parasite. The currently accessible over-the-counter drugs for disease management often present affordability challenges for the average person, exacerbated by the parasite's increasing resistance to them. This study investigated the phytoconstituents present in the ethyl acetate fraction of (EFSF) and explored the antimalarial effects of EFSF on mice infected with . Standard methods and gas chromatography-mass spectrometry (GC-MS) were used to identify phytoconstituents. Chloroquine phosphate-sensitive (NK-65) was intraperitoneally inoculated into Swiss mice. The antimalarial activity of EFSF was assessed at dose levels of 250, 500, and 750 mg/kg, using 4-day suppressive and curative antimalarial models. Parameters evaluated in the inoculated mice included rectal temperature (RT), body weight (BW), packed cell volume (PCV), level of parasitemia, and mean survival time (MST). Steroids, alkaloids, flavonoids, tannins, saponins, terpenoids, and cardiac glycosides were the identified phytochemicals present in EFSF, and GC-MS alongside reveals the presence of 20 bioactive compounds predominantly fatty acids and alcohol esters. Significant prevention of reductions in RT, BW, and PCV was observed in the EFSF-treated groups dose dependently relative to the untreated group. In addition, EFSF-treated groups significantly ( < 0.05) suppressed parasitemia and exhibited chemosuppression of 79.46% and 77.38% in 4-day suppressive, whereas suppression of 59.74% and 58.66% in curative treatment, respectively, at 500 and 750 mg/kg thus consequently extending the MST of infected treated mice compared with the untreated group. Put together, the EFSF exhibited enhanced antimalarial efficacy against mice infected with thus affirming that plants still maintain lead way as a potential source of novel antimalarial remedies.
疟疾在热带非洲国家构成了危及生命的风险，这源于疟原虫物种的感染。该地区自然资源丰富，拥有大量多样且大多未被探索的植物，这些植物具有控制这种原生动物寄生虫的潜力。目前可在柜台购买的用于疾病管理的药物，对于普通人来说往往存在可承受性挑战，而寄生虫对这些药物的耐药性不断增强，这一情况更加恶化。本研究调查了[植物名称]乙酸乙酯提取物（EFSF）中的植物成分，并探究了EFSF对感染[疟原虫名称]的小鼠的抗疟作用。采用标准方法和气相色谱 - 质谱联用（GC - MS）来鉴定植物成分。将对磷酸氯喹敏感的[疟原虫名称]（NK - 65）腹腔接种到瑞士小鼠体内。使用4天抑制性和治愈性抗疟模型，在250、500和750 mg/kg的剂量水平下评估EFSF的抗疟活性。对接种小鼠评估的参数包括直肠温度（RT）、体重（BW）、红细胞压积（PCV）、疟原虫血症水平和平均存活时间（MST）。甾体、生物碱、黄酮类、单宁、皂苷、萜类和强心苷是在EFSF中鉴定出的植物化学物质，GC - MS还显示存在20种生物活性化合物，主要是脂肪酸和醇酯。与未处理组相比，在EFSF处理组中观察到剂量依赖性地显著预防了RT、BW和PCV的降低。此外，EFSF处理组在4天抑制性治疗中分别在500和750 mg/kg剂量下显著（P <0.05）抑制了疟原虫血症，化学抑制率分别为79.46%和77.38%，而在治愈性治疗中抑制率分别为59.74%和58.66%，因此与未处理组相比，延长了感染处理小鼠的MST。综上所述，EFSF对感染[疟原虫名称]小鼠表现出增强的抗疟功效从而证实植物仍然作为新型抗疟药物潜在来源保持领先地位。
