[免疫吸附法用于分析细胞内病毒核糖核蛋白中的流感病毒RNA]
[The use of immunosorption for the analysis of influenza virus RNA in intracellular viral ribonucleoproteins].
作者信息
Sklianskaia E I, Varich N L, Amvros'eva T V, Kaverin N V
出版信息
Mol Gen Mikrobiol Virusol. 1985 Nov(11):36-7.
PMID:3916213
Abstract
Protein A-containing formaldehyde-fixed S. aureus (strain Cowan) was incubated with an antiviral serum or with a monospecific serum against NP protein, washed, and used as immunosorbent in order to isolate viral ribonucleoproteins (nucleocapsids) containing intact viral RNA from the extracts of influenza virus infected [3H]-uridine-labelled cells.
摘要
将含蛋白A的甲醛固定金黄色葡萄球菌（考恩菌株）与抗病毒血清或抗核蛋白（NP）蛋白的单特异性血清一起孵育，洗涤后用作免疫吸附剂，以便从感染流感病毒的[³H] - 尿苷标记细胞提取物中分离出含有完整病毒RNA的病毒核糖核蛋白（核衣壳）。
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