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鹰嘴豆(L.)分离株对杀菌剂的抗性。

Resistance of isolates from chickpeas ( L.) to fungicides.

作者信息

Endes Ali, Yones Amin Mohammed, Atmaca Sevim, Tahir Muhidin, Kayim Mukaddes

机构信息

Department of Plant Protection, Faculty of Agriculture, Yozgat Bozok University, Yozgat, Turkey.

Department of Horticultural Sciences, College of Agriculture, Oda Bultum University, P.O. Box 226, Chiro, Ethiopia.

出版信息

Heliyon. 2024 Aug 3;10(15):e35795. doi: 10.1016/j.heliyon.2024.e35795. eCollection 2024 Aug 15.

DOI:10.1016/j.heliyon.2024.e35795
PMID:39170508
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11337017/
Abstract

Ascochyta blight is a disease that causes significant yield losses in chickpea crops in Turkey under favorable environmental conditions. The fungal pathogen is the causative agent of this disease. The antifungal activity of previous fungicides against was not effective due to the heterothallic nature of the fungus. The aim of this study was to determine the sensitivity of to fungicides (25.2 g kg- boscalid + 12.8 g kg- pyraclostrobin; 50 % tebuconazole + 25 % trifloxystrobin; 62.5 g L- propiconazole + 37.5 g L- azoxystrobin; 80 % thiram; 80 % kükürt (sulphur); 80 % mancozeb; 80 % maneb) under and field conditions. Pure cultures of were isolated from infected chickpea plants collected in Boğazlayan, Sarıkaya, Sorgun, Merkez and Yerköy. A total of 14 isolates and 4 references were evaluated. The field test was conducted at Yozgat Bozok University, Yerköy Agricultural Application and Research Center Station. The trials began on March 14, 2021. The experimental area was divided into plots and the susceptible chickpea variety Sarı98 was used for the study. Two artificial inoculations were carried out approximately on the 40th and 80th days after sowing. Twenty-four hours after inoculation, the chickpea plants were sprayed with the fungicides Nativo® WG 75, Bellis®, Dikotan® M45 and Thiovit Jet® using a handheld sprayer. testing revealed that was resistant to kükürt (sulphur), thiram, maneb, and mancozeb. A field study showed that the percentage of isolates treated with the mancozeb fungicide was between 14 and 21 % of the control. Therefore, effective disease management strategies should include not only the use of fungicides, but also alternative approaches such as the use of resistant varieties. Moreover, the study focused on phenotypic resistance and suggests that future research should investigate the genetic and molecular mechanisms underlying resistance to enable better resistance management.

摘要

壳二孢叶枯病是一种在有利环境条件下会导致土耳其鹰嘴豆作物严重减产的病害。这种真菌病原体是该病害的致病因子。由于该真菌的异宗配合特性,先前的杀菌剂对其抗真菌活性并不有效。本研究的目的是确定壳二孢叶枯病菌在室内和田间条件下对杀菌剂(25.2克/千克肟菌酯+12.8克/千克吡唑醚菌酯;50%戊唑醇+25%肟菌酯;62.5克/升丙环唑+37.5克/升嘧菌酯;80%福美双;80%硫磺;80%代森锰锌;80%代森锰)的敏感性。壳二孢叶枯病菌的纯培养物从在博加兹拉扬、萨勒卡亚、索尔贡、梅尔凯兹和耶尔科伊采集的受感染鹰嘴豆植株中分离得到。总共对14个壳二孢叶枯病菌分离株和4个参照菌株进行了评估。田间试验在约兹加特博佐克大学耶尔科伊农业应用与研究中心站进行。试验于2021年3月14日开始。试验区被划分成小区,研究使用了感病鹰嘴豆品种Sarı98。在播种后大约第40天和第80天进行了两次人工接种。接种24小时后,使用手持喷雾器用杀菌剂拿敌稳®WG 75、百理通®、敌力脱®M45和硫悬浮剂®对鹰嘴豆植株进行喷雾。室内测试表明壳二孢叶枯病菌对硫磺、福美双、代森锰和代森锰锌具有抗性。一项田间研究表明,用代森锰锌杀菌剂处理的壳二孢叶枯病菌分离株的比例在对照的14%至21%之间。因此,有效的病害管理策略不仅应包括使用杀菌剂,还应包括使用抗性品种等替代方法。此外,该研究聚焦于表型抗性,并建议未来的研究应调查壳二孢叶枯病菌抗性的遗传和分子机制,以实现更好的抗性管理。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f7d/11337017/0ece0ebdbcd0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f7d/11337017/0ece0ebdbcd0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f7d/11337017/0ece0ebdbcd0/gr1.jpg