State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang 550004, China.
Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM, Guizhou Medical University, Guiyang 550004, China.
Int J Biol Macromol. 2024 Oct;278(Pt 3):134924. doi: 10.1016/j.ijbiomac.2024.134924. Epub 2024 Aug 22.
Selenium nanoparticles (SeNPs), as a potential cancer therapeutic agent, have attracted extensive attention due to their high anticancer activity and low toxicity. Polysaccharides could be the modifiers and stabilizers to improve the stability and dispersibility of SeNPs in aqueous solution. This study aimed to investigate the physicochemical characterization, stability, and anti-pancreatic cancer cell activities of SeNPs stabilized by a heteroxylan PVP3-1 extracted from the clusters of Prunella vulgaris Linn. Our results showed that PVP3-1 with Mw of 154 kDa was composed of →4)-β-D-Xylp(1→, →2, 4)-β-D-Xylp(1→, t-α-L-Araf(1→ and 4-MeO-α-D-GlcpA(1→. Red, zero-valent, and uniform spherical SeNPs with an average diameter of about 60 nm and high stability in aqueous solution were constructed successfully by polysaccharide PVP3-1. Anti-pancreatic cancer cell activity assays showed that PVP3-1-SeNPs could inhibit the proliferation and migration of pancreatic cancer cells in vitro. Furthermore, PVP3-1-SeNPs induced apoptosis and autophagy of pancreatic cancer cells through inhibiting mTOR signaling pathway. In conclusion, these results indicated that PVP3-1-SeNPs could be potential anti-tumor nanoparticles for treating pancreatic cancer.
硒纳米粒子(SeNPs)作为一种潜在的癌症治疗剂,由于其高抗癌活性和低毒性而引起了广泛关注。多糖可以作为修饰剂和稳定剂,以提高 SeNPs 在水溶液中的稳定性和分散性。本研究旨在研究由夏枯草簇中提取的异木聚糖 PVP3-1 稳定的 SeNPs 的理化特性、稳定性和抗胰腺癌细胞活性。我们的结果表明,Mw 为 154 kDa 的 PVP3-1 由→4)-β-D-Xylp(1→、→2、4)-β-D-Xylp(1→、t-α-L-Araf(1→和 4-MeO-α-D-GlcpA(1→组成。成功构建了由多糖 PVP3-1 稳定的红色、零价、均匀球形 SeNPs,其平均直径约为 60nm,在水溶液中具有高稳定性。体外抗胰腺癌细胞活性试验表明,PVP3-1-SeNPs 可以抑制胰腺癌细胞的增殖和迁移。此外,PVP3-1-SeNPs 通过抑制 mTOR 信号通路诱导胰腺癌细胞凋亡和自噬。总之,这些结果表明 PVP3-1-SeNPs 可能是治疗胰腺癌的潜在抗肿瘤纳米粒子。