State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of International Education, Northwest A&F University, Yangling 712100, China.
J Agric Food Chem. 2024 Sep 4;72(35):19295-19303. doi: 10.1021/acs.jafc.4c04091. Epub 2024 Aug 23.
As a kind of obligate biotrophic fungus, f. sp. () secretes vast effectors via haustoria to host cells during the infection to inhibit host defense responses and promote fungal invasion. In this study, based on the completion of genome sequencing and haustorial transcriptome sequencing of , we identified a effector (Hasp155) that is significantly induced in the early stage of infection to wheat. The 18 N-terminal amino acids of Hasp155 encoded a signal peptide with a secretory function. Transient expression of 155 in inhibited Bax-induced cell death as well as chitin-triggered callose deposition and defense-related gene expression. Moreover, delivery of the Hasp155 protein into wheat cells via type three secretion systems (TTSS) led to reduced plant immunity to nonpathogenic bacteria and to the avirulent race with decreased HO accumulation and promoted development. Furthermore, transgenic overexpression of 155 significantly renders wheat resistance susceptible, resulting in a decreased defense response and increased pathogenicity. Overall, these results indicate that Hasp155 is an important effector of pathogenicity by suppressing plant immunity.
作为一种专性活体营养真菌，（）在侵染宿主细胞的过程中通过吸器分泌大量效应子，以抑制宿主防御反应并促进真菌入侵。在本研究中，基于（）基因组测序和吸器转录组测序的完成，我们鉴定了一个在小麦（）侵染早期显著诱导的效应子（Hasp155）。Hasp155 的 18 个 N 端氨基酸编码了一个具有分泌功能的信号肽。瞬时表达 155 可抑制 Bax 诱导的细胞死亡以及几丁质触发的胼胝质沉积和防御相关基因的表达。此外，通过 III 型分泌系统（TTSS）将 Hasp155 蛋白递送到小麦细胞中，导致对非致病性细菌的植物免疫力降低，对无毒（）小种的侵染性降低，HO 积累减少，促进了（）的发育。此外，155 的过表达转基因小麦对感病性显著敏感，导致防御反应降低和致病性增加。总体而言，这些结果表明 Hasp155 通过抑制植物免疫来抑制植物免疫，是（）致病性的一个重要效应子。
