Gschwendt M, Kittstein W, Horn F, Marks F
Biochem Biophys Res Commun. 1985 Jan 16;126(1):327-32. doi: 10.1016/0006-291x(85)90609-6.
The antilymphocytic and antiphlogistic agent cyclosporin A (CsA) inhibits in vivo various effects induced by the tumor promoting phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA). These include the edema of the mouse ear, the alkaline phosphatase (AP) activity and the ornithine decarboxylase (ODC) activity in mouse epidermis as well as the generation of a specific arachidonic acid (AA) metabolite in mouse epidermis. AA metabolism in an epidermal cell-free system of mouse epidermis was not suppressed by CsA. According to thin layer chromatography the TPA-induced and as yet unidentified AA metabolite exhibits a polarity between that of 5-HETE and 12-/15-HETE. Studies with inhibitors indicate it to be a lipoxygenase product.
抗淋巴细胞及抗炎药物环孢菌素A（CsA）在体内可抑制由促肿瘤佛波酯12-O-十四酰佛波醇-13-乙酸酯（TPA）诱导的多种效应。这些效应包括小鼠耳部水肿、小鼠表皮中的碱性磷酸酶（AP）活性和鸟氨酸脱羧酶（ODC）活性，以及小鼠表皮中一种特定花生四烯酸（AA）代谢产物的生成。CsA并未抑制小鼠表皮无细胞体系中的AA代谢。根据薄层色谱分析，TPA诱导产生的且尚未鉴定的AA代谢产物的极性介于5-羟二十碳四烯酸（5-HETE）和12-/15-羟二十碳四烯酸（12-/15-HETE）之间。用抑制剂进行的研究表明它是一种脂氧合酶产物。
