Lemon zinc finger protein ClSUP induces accumulation of reactive oxygen species and inhibits citrus yellow vein-clearing virus infection via interactions with ClDOF3.4.

Citrus yellow vein-clearing virus (Potexvirus citriflavivenae; CYVCV) is an increasing threat to citrus cultivation. Notably, the role of zinc finger proteins (ZFPs) in mediating viral resistance in citrus plants is unclear. In this study, we demonstrated that ZFPs ClSUP and ClDOF3.4 enhanced citrus defense responses against CYVCV in Eureka lemon (Citrus limon 'Eureka'). ClSUP interacted with the coat protein (CP) of CYVCV to reduce CP accumulation and inhibited its silencing suppressor function. Overexpression of CISUP triggered reactive oxygen species (ROS) and salicylic acid (SA) pathways, and enhanced resistance to CYVCV infection. In contrast, ClSUP silencing resulted in increased CP accumulation and down-regulated ROS and SA-related genes. ClDOF3.4 interacted with ClSUP to facilitate its interactions with CP. Furthermore, ClDOF3.4 synergistically regulated the accumulation of ROS and SA with ClSUP and accelerated down-regulation of CP accumulation. Transgenic plants co-expressing ClSUP and ClDOF3.4 significantly decreased the CYVCV. These findings provide a new reference for understanding the interaction mechanism between the host and CYVCV.