Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
National Innovation Centre for Synthetic Biology, Tianjin 300308, China.
J Agric Food Chem. 2024 Sep 4;72(35):19462-19469. doi: 10.1021/acs.jafc.4c06071. Epub 2024 Aug 26.
Sustainable production of edible microbial proteins and red food colorants is an important demand for future food. Therefore, creation of a chassis strain that can efficiently synthesize both products is extremely necessary and meaningful. To realize this envision, a CRISPR/Cas9-based visual multicopy integration system was successfully developed in . Subsequently, the synthesis of the red food colorant betanin was achieved in the engineered using the above system. After fermentation optimization, the final yields of betanin and mycoprotein reached 1.91 and 9.53 g/L, respectively, when the constant pH naturally decreased from 6 to 4 without the addition of acid after 48 h of fermentation. These results determine a highly suitable chassis strain for the microbial biomanufacturing of betanin, and the obtained engineered strain here is expected to expand the application prospect and improve economic returns of in the field of future food.
可食用微生物蛋白和红色食品着色剂的可持续生产是未来食品的重要需求。因此，创造一种能够高效合成这两种产品的底盘菌株是极其必要和有意义的。为了实现这一设想，在 中成功开发了基于 CRISPR/Cas9 的可视化多拷贝整合系统。随后，利用上述系统在工程 中实现了红色食品着色剂甜菜红素的合成。经过发酵优化，当发酵 48 小时后，恒定 pH 值自然从 6 下降到 4 而无需添加酸时，甜菜红素和菌蛋白的最终产量分别达到 1.91 和 9.53 g/L。这些结果确定了一种非常适合微生物生物制造甜菜红素的底盘菌株，并且这里获得的工程菌株有望扩大 在未来食品领域的应用前景和提高经济效益。
