Chang Xiaoyu, Wang Yuyan, Zain Abbas, Yu Haibing, Huang Weidong
College of Resources and Environment, Anhui Science and Technology University, Chuzhou 233100, China.
College of Agriculture, Anhui Science and Technology University, Chuzhou 233100, China.
J Fungi (Basel). 2024 Jul 25;10(8):519. doi: 10.3390/jof10080519.
Difenoconazole-loaded (CS-DIF) microcapsules were synthesized by encapsulating difenoconazole into biocompatible chitosan. The physical and chemical properties indicated that the encapsulation and chemical loading rates were 85.58% and 61.98%, respectively. The microcapsules exhibited prominent controlled-release and surface stability performance. The cumulative release rate was only 33.6% in 168 h, and the contact angle decreased by 11.73° at 120 s compared with difenoconazole. The antifungal activity of the CS-DIF microcapsules against was confirmed through observations of colony growth, in vitro and in vivo inoculation, mycelium morphology, as well as DNA and protein leakage. The antioxidant enzyme activity of superoxide dismutase, peroxidase, and catalase decreased by 65.1%, 84.9%, and 69.7%, respectively, when was treated with 200 μg/mL microcapsules, compared with the control in 24 h. The enzymatic activity of polyphenol oxidase decreased by 323.8%. The reactive oxygen species contents of hydrogen peroxide and superoxide anions increased by 204.6% and 164%, respectively. Additionally, the soluble sugar and soluble protein contents decreased by 65.5% and 69.6%, respectively. These findings provided a novel approach to control the growth of efficiently, laying a foundation for reducing the quantity and enhancing the efficiency of chemical pesticides. The CS-DIF microcapsules exhibited a strong inhibitory effect on fungus, effectively preventing and controlling leaf spot disease and showing potential for field applications. This study might be of great significance in ensuring plant protection strategies.
通过将苯醚甲环唑包封到生物相容性壳聚糖中，合成了负载苯醚甲环唑的（壳聚糖 - 苯醚甲环唑）微胶囊。物理和化学性质表明，包封率和化学负载率分别为85.58%和61.98%。微胶囊表现出显著的控释和表面稳定性性能。在168小时内累积释放率仅为33.6%，与苯醚甲环唑相比，在120秒时接触角降低了11.73°。通过观察菌落生长、体外和体内接种、菌丝形态以及DNA和蛋白质泄漏，证实了壳聚糖 - 苯醚甲环唑微胶囊对[具体真菌名称未给出]的抗真菌活性。与24小时的对照相比，当用200μg/mL微胶囊处理[具体对象未给出]时，超氧化物歧化酶、过氧化物酶和过氧化氢酶的抗氧化酶活性分别降低了65.1%、84.9%和69.7%。多酚氧化酶的酶活性降低了323.8%。过氧化氢和超氧阴离子的活性氧含量分别增加了204.6%和164%。此外，可溶性糖和可溶性蛋白质含量分别降低了65.5%和69.6%。这些发现为有效控制[具体对象未给出]的生长提供了一种新方法，为减少化学农药用量和提高效率奠定了基础。壳聚糖 - 苯醚甲环唑微胶囊对真菌表现出强烈的抑制作用，有效预防和控制叶斑病，并显示出田间应用的潜力。这项研究在确保植物保护策略方面可能具有重要意义。
