Tian Wen-Hui, Jin Yan, Liao Yue-Chi, Faraj Turki Kh, Guo Xin-Yong, Maharachchikumbura Sajeewa S N
Center for Informational Biology, College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, China.
Department of Soil Science, College of Food and Agriculture Sciences, King Saud University, P.O. Box 145111, Riyadh 11362, Saudi Arabia.
J Fungi (Basel). 2024 Aug 2;10(8):542. doi: 10.3390/jof10080542.
Pleosporales is a highly diverse (and the largest) order in Dothideomycetes, and it is widespread in decaying plants in various environments around the world. During a survey of fungal diversity in Sichuan Province, China, specimens of hyphomycetous and -like fungi were collected from dead branches of pine trees and cherry trees. These taxa were initially identified as members of Massarinaceae and Thyridariaceae through morphological examination. Phylogenetic analyses of the Thyridariaceae, combining ITS, SSU, LSU, , and sequence data, indicated a distinct clade sister to and , distinct from any genus in the family. Thus, a new genus, , is proposed to accommodate the type species , which is characterized by semi-immersed globose to oblong ascomata with an ostiolar neck, cylindrical to clavate asci with an ocular chamber, and hyaline to dark brown, fusiform, 3-5-transversely septate ascospores with an inconspicuous mucilaginous sheath. Based on the morphological comparisons and multi-locus phylogenetic analyses (ITS, SSU, LSU, , and ) of the Massarinaceae, we have identified three collections belonging to the genus , leading us to propose sp. nov., sp. nov., and as a new host record. According to Phylogenetic analysis, formed an independent clade sister to and , and represents the closest sister clade to . is distinct from by the shorter conidiophores and by the longer and wider conidia. The differs from in having longer conidiophores and smaller conidia. This study extends our understanding of diversity within Thyridariaceae and . Our findings underscore the rich biodiversity and potential for discovering novel fungal taxa within these groups.
格孢腔菌目是座囊菌纲中一个高度多样化（也是最大的）目，广泛分布于世界各地各种环境中的腐烂植物上。在中国四川省进行真菌多样性调查期间，从松树和樱桃树的枯枝上采集了丝孢菌和类似丝孢菌的真菌标本。通过形态学检查，这些分类单元最初被鉴定为日规菌科和格孢腔菌科的成员。结合ITS、SSU、LSU和 序列数据对格孢腔菌科进行系统发育分析，结果表明存在一个与 和 为姐妹群的独特分支，与该科中的任何属都不同。因此，提出了一个新属 ，以容纳模式种 ，其特征为半埋生的球形至椭圆形子囊果，具孔口颈，具眼腔的圆柱形至棒状子囊，以及透明至深褐色、梭形、3-5个横向隔膜的子囊孢子，具不明显的粘液鞘。基于对日规菌科的形态比较和多位点系统发育分析（ITS、SSU、LSU、 和 ），我们鉴定出三个属于 属的样本，从而提出 新种、 新种，并将 作为新的寄主记录。根据系统发育分析， 形成了一个与 和 为姐妹群的独立分支， 代表了与 最接近的姐妹分支。 与 的区别在于分生孢子梗较短，与 的区别在于分生孢子更长更宽。 与 的区别在于分生孢子梗更长，分生孢子更小。本研究扩展了我们对格孢腔菌科和 内多样性的认识。我们的发现强调了这些类群中丰富的生物多样性以及发现新真菌分类单元的潜力。
