Shaanxi Engineering Laboratory for Food Green Processing and Safety Control, and Shaanxi Key Laboratory for Hazard Factors Assessment in Processing and Storage of Agricultural Products, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, China.
Key Laboratory of Yunnan University for Plateau Characteristic Functional Food, School of Chemistry and Chemical Engineering, Zhaotong University, 657000, China.
Food Chem. 2024 Dec 15;461:140934. doi: 10.1016/j.foodchem.2024.140934. Epub 2024 Aug 22.
Fu-brick tea (FBT) is predominately fermented by Eurotium Cristatum, FBT polysaccharides (FTPs) and Eurotium Cristatum extracellular polysaccharides (ECPs) are the main active substances in FBT and Eurotium Cristatum, respectively. FTPs was shown to exhibit higher levels of uronic acids, proteins, and polyphenols as compared to ECPs (p < 0.05), contributing to the superior antioxidant activity observed in FTPs. Additionally, FTPs had better water solubility and thermal stability than ECPs. Interestingly, in vitro digestive simulation revealed that FTPs and ECPs resist digestion in the stomach and small intestine. Excitingly, utilizing in vitro fermentation with feces from healthy individuals and type 2 diabetes mellitus (T2DM) patients demonstrated that FTPs and ECPs promote the production of SCFAs. Still, FTPs resulted in greater SCFAs contents than ECPs (p < 0.05). Moreover, FTPs and ECPs fermentation by T2DM patients' fecal microbiota affected different metabolomic pathways. Our findings suggested that FTPs holds great promise for application in functional foods.
茯砖茶（FBT）主要由黑曲霉发酵而成，FBT 多糖（FTPs）和黑曲霉胞外多糖（ECPs）分别是 FBT 和黑曲霉中的主要活性物质。FTPs 中的酸性多糖、蛋白质和多酚含量均高于 ECPs（p<0.05），这使其具有更高的抗氧化活性。此外，FTPs 的水溶性和热稳定性优于 ECPs。有趣的是，体外消化模拟表明，FTPs 和 ECPs 可抵抗胃和小肠的消化。令人兴奋的是，利用健康个体和 2 型糖尿病（T2DM）患者的粪便进行体外发酵实验表明，FTPs 和 ECPs 可促进短链脂肪酸（SCFAs）的生成。但 FTPs 产生的 SCFAs 含量高于 ECPs（p<0.05）。此外，FTPs 和 ECPs 经 T2DM 患者粪便微生物群发酵后，会影响不同的代谢途径。我们的研究结果表明，FTPs 在功能性食品中有很大的应用潜力。
