Department of Technology and Food Assessment, Division of Milk Technology, Institute of Food Sciences, Warsaw University of Life Sciences-SGGW, 02-787 Warsaw, Poland.
Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, 02-787 Warsaw, Poland.
Int J Mol Sci. 2024 Aug 7;25(16):8622. doi: 10.3390/ijms25168622.
are environmental commensals that have not been associated with any clinical infections. To date, they are the least understood and described species within the genus. The objective of this study was to use a draft genome sequence (DGS) of the strain s37 to screen for genes encoding for antibiotic resistance, virulence, response to environmental stress, and biofilm formation. The strain was isolated in Poland from commercial small radish sprouts. This is the second genome of this species available in the GenBank database. The comparative genome analysis (cgMLST) of s37 with other spp. including the pathogenic species and the plant-associated closely related genera and was also performed. The assembled and annotated genome of the s37 genome was 4,590,991 bp in length, with a total gene number of 4384, and a GC content of 55.7%. The s 37 genome encoded for genes associated with resistance to stressful environmental conditions (metal resistance genes: zinc, copper, osmotic regulation, and desiccation stress), 17 antimicrobial resistance genes encoding resistance to various classes of antibiotics and 50 genes encoding for the virulence factors. The latter were mainly genes associated with adhesion, chemotaxis, hemolysis, and biofilm formation. Cg-MLST analysis (3991 genes) revealed a greater similarity of s37 to , , and than to other species of the genus . Studies on the diversity, pathogenicity, and virulence of species isolated from different sources are still insufficient and should certainly be continued. Especially the analysis of rare strains such as s37 is very important because it provides new information on the evolution of these bacteria. Comparative cgMLST analysis of s37 with other species, as well as closely related genera and , complements the knowledge on their adaptability to specific environments such as desiccation.
它们是与任何临床感染都无关的环境共生菌。迄今为止，它们是该属中了解和描述最少的物种。本研究的目的是利用 菌株 s37 的草图基因组序列 (DGS) 筛选编码抗生素抗性、毒力、对环境压力的反应和生物膜形成的基因。该菌株是从波兰的商业小萝卜芽中分离出来的。这是该物种在 GenBank 数据库中的第二个基因组。还对 s37 与其他 spp.（包括致病菌 和与植物密切相关的属 和 ）进行了比较基因组分析 (cgMLST)。s37 的组装和注释基因组长度为 4,590,991 bp，总基因数为 4384，GC 含量为 55.7%。s37 基因组编码与抵抗应激环境条件相关的基因（耐金属基因：锌、铜、渗透压和干燥胁迫）、17 个编码各种抗生素类别的抗药性基因和 50 个编码毒力因子的基因。后者主要与粘附、趋化性、溶血和生物膜形成相关的基因。cg-MLST 分析（3991 个基因）显示，s37 与 、 、 和 更为相似，而与该属的其他物种则不太相似。对从不同来源分离的 物种的多样性、致病性和毒力的研究仍然不足，当然应该继续进行。特别是对 s37 等稀有菌株的分析非常重要，因为它提供了有关这些细菌进化的新信息。s37 与其他 物种以及与植物密切相关的属 进行的比较 cgMLST 分析，补充了它们对干燥等特定环境的适应性的知识。
