Dees C, Wade W F, German T L, Marsh R F
J Gen Virol. 1985 Apr;66 ( Pt 4):845-9. doi: 10.1099/0022-1317-66-4-845.
The sensitivity of the scrapie agent to u.v. inactivation was found to be related to the purity of the tissue preparation. Scrapie infectivity associated with membrane vesicles was unaffected when irradiated with 10(4) J/m2. Irradiation of more highly purified preparations from detergent-extracted CsCl gradient fractions reduced scrapie infectivity from 10(7.8) log10 LD50 per ml to as low as 10(4.5). Sensitivity of membrane-associated scrapie infectivity to inactivation by u.v. irradiation could be increased by addition of chlorpromazine, a phenthiazine antipsychotic which penetrates lipid bilayers and induces single-strand breaks in nucleic acids under irradiation. Chlorpromazine without irradiation, and a semiquinone protein-binding radical of chlorpromazine, failed to decrease scrapie infectivity by themselves. A closely related phenthiazine antipsychotic, trifluoperazine, which does not bind to nucleic acids, did not reduce scrapie infectivity. These findings suggest that the target of u.v. radiation for inactivation of scrapie infectivity in the presence of chlorpromazine is an essential nucleic acid.
已发现羊瘙痒病病原体对紫外线灭活的敏感性与组织制剂的纯度有关。用10(4) J/m2照射时，与膜泡相关的羊瘙痒病传染性不受影响。用洗涤剂提取的CsCl梯度级分中更高纯度的制剂进行照射，可使羊瘙痒病传染性从每毫升10(7.8) log10 LD50降至低至10(4.5)。通过添加氯丙嗪可提高与膜相关的羊瘙痒病传染性对紫外线照射灭活的敏感性，氯丙嗪是一种吩噻嗪类抗精神病药物，可穿透脂质双层并在照射下诱导核酸单链断裂。未照射的氯丙嗪以及氯丙嗪的半醌蛋白结合自由基自身并不能降低羊瘙痒病传染性。一种密切相关的不与核酸结合的吩噻嗪类抗精神病药物三氟拉嗪，不能降低羊瘙痒病传染性。这些发现表明，在氯丙嗪存在的情况下，紫外线辐射使羊瘙痒病传染性失活的靶点是一种必需核酸。
