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用于控制玫瑰冠瘿病的化学和生物制品评估

Evaluation of Chemical and Biological Products for Control of Crown Gall on Rose.

作者信息

Oksel Cansu, Liyanapathiranage Prabha, Parajuli Madhav, Avin Farhat A, Jennings Christina, Simmons Terri, Baysal-Gurel Fulya

机构信息

Department of Agricultural Sciences and Engineering, College of Agriculture, Otis L. Floyd Nursery Research Center, Tennessee State University, McMinnville, TN 37110, USA.

出版信息

Pathogens. 2024 Aug 21;13(8):708. doi: 10.3390/pathogens13080708.

Abstract

Crown gall is a soil-borne bacterial disease caused by , leading to significant economic losses in many plant species. For the assessment of the biological and chemical products on crown gall, each plant's crown region and roots were wounded, and then were dipped into their respective treatments. After the treatments, the plants were inoculated with a suspension of pathogenic isolate FBG1034 and maintained in a greenhouse for six months to assess them for gall formation. A quantitative real-time PCR assay was performed to quantify the . using the gene. Biological products such as the strain K1026, and strains 1 and 2, resulted in the lowest average root gall diameter and significantly reduced the crown gall diameter to stem diameter ratio, and the chemical product copper octanoate reduced the number of crown and root galls as well as the crown and root gall diameter compared to the inoculated, non-treated control. Moreover, both the strain K1026 and strain 1 treatments resulted in an approximately 85% and 65% reduction in crown and root gall incidence, respectively, in both of the trials compared to the inoculated, non-treated plants. The findings of this study indicate that the use of biological and chemical products could help to suppress crown and root gall disease in rose plants.

摘要

冠瘿病是一种由土壤传播的细菌性疾病,由[具体病菌名称缺失]引起,会给许多植物物种造成重大经济损失。为了评估生物和化学产品对冠瘿病的防治效果,对每株植物的冠部区域和根部进行创伤处理,然后将其浸入各自的处理液中。处理后,用致病性分离株FBG1034的悬浮液对植物进行接种,并在温室中培养六个月,以评估瘿瘤形成情况。采用定量实时PCR分析方法,以[具体基因名称缺失]基因为参照来定量[具体物质名称缺失]。生物产品如[具体生物名称缺失]菌株K1026以及菌株1和菌株2,使平均根瘿直径最小,并显著降低了冠瘿直径与茎直径的比值,化学产品辛酸铜与接种但未处理的对照相比,减少了冠部和根部瘿瘤的数量以及冠部和根部瘿瘤的直径。此外,在两项试验中,与接种但未处理的植株相比,[具体生物名称缺失]菌株K1026和菌株1的处理分别使冠部和根部瘿瘤发病率降低了约85%和65%。本研究结果表明,使用生物和化学产品有助于抑制玫瑰植株的冠部和根部瘿瘤病。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a63/11357299/903fe195d019/pathogens-13-00708-g001a.jpg

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