Yu Wang, Zhou Xiangru, Xu Hongwei, Zhou Xiaofu
Jilin Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping 136000, China.
Plants (Basel). 2024 Aug 12;13(16):2232. doi: 10.3390/plants13162232.
Increased UV-B radiation due to ozone depletion adversely affects plants. This study focused on the metabolite dynamics of Pall. () and the role of ABA in mitigating UV-B stress. Chlorophyll fluorescence metrics indicated that both JA and ABA increased UV-B resistance; however, the effect of JA was not as strong as that of ABA. Metabolomic analysis using UPLC-MS/MS (ultra-performance liquid chromatography and tandem mass spectrometry) revealed significant fluctuations in metabolites under UV-B and ABA application. UV-B decreased amino acids and increased phenolics, suggesting antioxidant defense activation. ABA treatment upregulated lipids and phenolic acids, highlighting its protective role. Multivariate analysis showed distinct metabolic clusters and pathways responding to UV-B and ABA, which impacted amino acid metabolism and hormone signal transduction. Exogenous ABA negatively regulated the JA signaling pathway in UV-B-exposed , as shown by KEGG enrichment. This study deepens understanding of plant stress-tolerance mechanisms and has implications for enhancing plant stress tolerance through metabolic and hormonal interventions.
由于臭氧消耗导致的紫外线B辐射增加对植物产生不利影响。本研究聚焦于 Pall. () 的代谢物动态以及脱落酸在减轻紫外线B胁迫中的作用。叶绿素荧光指标表明茉莉酸和脱落酸均增强了对紫外线B的抗性；然而，茉莉酸的效果不如脱落酸显著。使用超高效液相色谱 - 串联质谱法（UPLC-MS/MS）进行的代谢组学分析揭示了在紫外线B照射和脱落酸处理下代谢物的显著波动。紫外线B降低了氨基酸含量并增加了酚类物质，表明抗氧化防御被激活。脱落酸处理上调了脂质和酚酸，突出了其保护作用。多变量分析显示了对紫外线B和脱落酸有不同反应的代谢簇和途径，这影响了氨基酸代谢和激素信号转导。如KEGG富集所示，外源脱落酸在紫外线B照射下的 中对茉莉酸信号通路起负调控作用。本研究加深了对植物胁迫耐受机制的理解，并对通过代谢和激素干预提高植物胁迫耐受性具有启示意义。
