Wang Shijie, Chen Keke, Guo Jiaqi, Zhang Panwang, Li Yuchen, Xu Zhenghao, Cui Langjun, Qiang Yi
National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an 710119, China.
Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, Shaanxi Normal University, Xi'an 710119, China.
Plants (Basel). 2024 Aug 12;13(16):2236. doi: 10.3390/plants13162236.
P. Y. Li () is a traditional Chinese herbal medicine that has been used for over two millennia to treat cough and expectoration. However, the increasing cultivation of has led to the spread of bulb rot diseases. In this study, pathogens were isolated from rotten bulbs. Through molecular identification, pathogenicity testing, morphological assessment, and microscopy, was identified as the pathogen causing bulb rot in . The colonization of in the bulbs was investigated through microscopic observation. The rapid and accurate detection of this pathogen will contribute to better disease monitoring and control. Loop-mediated isothermal amplification (LAMP) and qPCR methods were established to quickly and specifically identify this pathogen. These results provide valuable insights for further research on the prediction, rapid detection, and effective prevention and control of bulb rot in .
梨叶蓼是一种已经被用于治疗咳嗽和祛痰两千多年的传统中草药。然而，梨叶蓼种植量的增加导致了球茎腐烂病的传播。在本研究中，从腐烂的梨叶蓼球茎中分离出病原体。通过分子鉴定、致病性测试、形态学评估和显微镜观察，确定该病原体为导致梨叶蓼球茎腐烂的病原菌。通过显微镜观察研究了该病原菌在球茎中的定殖情况。该病原菌的快速准确检测将有助于更好地进行病害监测和防控。建立了环介导等温扩增（LAMP）和qPCR方法以快速、特异性地鉴定该病原菌。这些结果为进一步研究梨叶蓼球茎腐烂的预测、快速检测及有效防控提供了有价值的见解。
