Liu Renjian, Liu Hongmei, Wang Yuyuan, Chen Jiahao, Qiu Zihao, Zheng Yanchun, Sun Binmei, Tan Xindong, Shu Canwei, Liu Shaoqun, Zheng Peng
Department of Tea, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
Plants (Basel). 2024 Aug 20;13(16):2321. doi: 10.3390/plants13162321.
Sooty mould (SM) disease affects the growth, development and metabolism of plants and reduces the commodity and economic value of crops. SM disease is one of the important leaf diseases in tea plants. Nonetheless, studies on the effect of SM disease in tea plants are rare. Herein, we found that SM disease disrupted the cell morphology and structure and reduced the contents of caffeine, theanine, and catechins in the mature leaves of tea plants. Transcriptome analysis revealed that SM disease inhibited the biosynthesis of lignin, chlorophyll, catechin, caffeine, and theanine and affected the plant-pathogen interactions in the mature leaves of tea plants by downregulating gene expression. In addition, two fungal isolates, MTzyqA and MTzyqB, were obtained from the mature leaves of diseased tea plants. These strains were identified as by mulitgene phylogenetic analysis, and they grew epiphytically on the leaves of tea plants. The biocontrol bacteria JT68, ZGT5, and BX1 had obvious inhibitory effect on MTzyqA and MTzyqB. These results provide a basis for understanding the effect of SM disease in tea plants.
煤烟病影响植物的生长、发育和新陈代谢，降低作物的商品价值和经济价值。煤烟病是茶树重要的叶部病害之一。然而，关于煤烟病对茶树影响的研究却很少。在此，我们发现煤烟病破坏了茶树成熟叶片的细胞形态和结构，并降低了咖啡因、茶氨酸和儿茶素的含量。转录组分析表明，煤烟病通过下调基因表达抑制了茶树成熟叶片中木质素、叶绿素、儿茶素、咖啡因和茶氨酸的生物合成，并影响了植物与病原体的相互作用。此外，从患病茶树的成熟叶片中获得了两株真菌分离物MTzyqA和MTzyqB。通过多基因系统发育分析鉴定这些菌株，它们在茶树叶片上附生生长。生防细菌JT68、ZGT5和BX1对MTzyqA和MTzyqB有明显的抑制作用。这些结果为了解煤烟病对茶树的影响提供了依据。
