Parasitic and Honeybee Disease Laboratory, Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea; Faculty of Biotechnology, Thai Nguyen University of Sciences, Thai Nguyen 250000, Vietnam.
Parasitic and Honeybee Disease Laboratory, Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea.
Vet Parasitol. 2024 Oct;331:110292. doi: 10.1016/j.vetpar.2024.110292. Epub 2024 Aug 23.
The poultry red mite (PRM), Dermanyssus gallinae, significantly impacts the health of egg-laying hens. Mites feed on the blood of infested chickens and have a great economic impact on the poultry industry. Chemical treatment of mites raises concerns about their resistance to miticides and residues in eggs and poultry. Biocontrol using entomopathogenic fungi is expected to be a chemical-free strategy for reducing PRM infestations. Therefore, the present study aimed to investigate the effects of various entomopathogenic fungal species collected in South Korea on the inhibition of PRM. Seventeen strains of six fungal species collected from various sources were used to evaluate acaricidal activity against PRM. The results showed that 16/17 strains had acaricidal properties against PRM, of which strains of Metarhizium anisopliae had the highest acaricidal activity. Mites treated with M. anisopliae CBNU 4-2 showed 100 % mortality 5 d after inoculation, followed by M. flavoviride var. pemphigi. The M. flavoviride var. pemphigi CBNU 1-1-1 showed 97.78 % mortality after 10 d of exposure to fungi. The mortality rate of PRM treated with other strains slowly increased and reached its highest value on the 14th day of inoculation. The results of this study provide information on the acaricidal activity of different entomopathogenic fungi against PRM. This information is important for the selection of fungal species for developing biocontrol methods for PRM treatment. These strains could be used for further evaluation of PRM treatment on chicken farms, or in combination with other methods, to increase PRM treatment efficiency.
鸡皮刺螨（PRM），又称鸡螨，对产蛋母鸡的健康有重大影响。螨虫以受感染鸡的血液为食，对家禽业有重大的经济影响。螨虫的化学处理引起了人们对杀螨剂抗性和鸡蛋及家禽中残留的关注。利用昆虫病原真菌进行生物防治有望成为一种减少 PRM 感染的无化学物质策略。因此，本研究旨在调查在韩国收集的各种昆虫病原真菌对抑制 PRM 的影响。从各种来源收集的六种真菌的 17 株菌株被用于评估对 PRM 的杀螨活性。结果表明，17/17 株菌株对 PRM 具有杀螨特性，其中绿僵菌（Metarhizium anisopliae）菌株的杀螨活性最高。用绿僵菌（Metarhizium anisopliae）CBNU 4-2 处理的螨虫在接种后 5 天内死亡率达到 100%，其次是绿僵菌（Metarhizium flavoviride var. pemphigi）。暴露于真菌 10 天后，绿僵菌（Metarhizium flavoviride var. pemphigi）CBNU 1-1-1 的死亡率达到 97.78%。用其他菌株处理的 PRM 死亡率缓慢增加，在接种后第 14 天达到最高值。本研究的结果提供了不同昆虫病原真菌对 PRM 的杀螨活性信息。这些信息对于选择用于开发 PRM 处理的生物防治方法的真菌物种很重要。这些菌株可用于进一步评估在养鸡场使用这些菌株治疗 PRM，或与其他方法结合使用，以提高 PRM 处理效率。
