Department of Mining & Metallurgical Engineering, Amirkabir University of Technology, Tehran, Iran; Department of Mining Engineering, Amirkabir University of Technology, 424, Hafez Avenue, Tehran 1875-4413, Iran.
Department of Mining & Metallurgical Engineering, Amirkabir University of Technology, Tehran, Iran; Department of Mining Engineering, Amirkabir University of Technology, 424, Hafez Avenue, Tehran 1875-4413, Iran.
Int J Biol Macromol. 2024 Nov;279(Pt 2):134944. doi: 10.1016/j.ijbiomac.2024.134944. Epub 2024 Aug 30.
In this study, alginate/vermiculite (Alg/VMT) hydrogel with 3-aminopropyl triethoxysilane (Alg/VSN) and tetraethoxysilane (Alg/VS) synthesized with various concentrations of CaCl (10 %-15 %-20 % M) to extend the release of 6-Aminopenicillanic acid (AP). Composites characterized by XRD, FTIR and BET. The result of Alg/VS composite shows an excellent loading of 243.90 mg/g through AP intercalated in the VMT layer. The equilibrium and Kinetic studies indicated that AP adsorption on Alg/VS and Alg/VSN was heterogeneous with chemical interaction. The in-vitro release Alg/VS showed a rapid burst release of 14 % in the first half an hour and only 75 % of the drug remained in the composite. Whereas, the in-vitro release Alg/VSN showed substantially less burst release with the cumulative release of 9 % (in the first 0.5 h). In-vitro release kinetics in the presence of CaCl concentrations showed that maximum 19 % of AP released within 12 h. The kinetic release was followed by a controlled release pattern (Korsmeyer-Peppas model) with Fick's law mechanism. The composites behaved as barriers against cell growth and had better biocompatibility against standard strains of Pseudomonas aeruginosa and Methicillin-Resistant Staphylococcus. MTT assay results from per cent cell viability composites modified by silanol groups were 96 % the means samples were nontoxic. The types of newly synthesized composites were able to finely decrease cell toxicity and improve AP release in vitro.
在这项研究中，合成了不同浓度 CaCl（10%-15%-20%M）的藻酸盐/蛭石（Alg/VMT）水凝胶，其中含有 3-氨丙基三乙氧基硅烷（Alg/VSN）和四乙氧基硅烷（Alg/VS），以延长 6-氨基青霉烷酸（AP）的释放。通过 XRD、FTIR 和 BET 对复合材料进行了表征。Alg/VS 复合材料的结果表明，AP 插层在 VMT 层中具有出色的 243.90mg/g 的负载量。平衡和动力学研究表明，AP 在 Alg/VS 和 Alg/VSN 上的吸附是异质的，存在化学相互作用。Alg/VS 的体外释放显示出前半小时快速释放 14%，而药物只有 75%残留在复合材料中。相比之下，Alg/VSN 的体外释放显示出明显较少的突释，累积释放量为 9%（在前 0.5 小时）。在存在 CaCl 浓度的情况下，体外释放动力学表明，AP 在 12 小时内最大释放 19%。释放动力学遵循控制释放模式（Korsmeyer-Peppas 模型）和菲克定律机制。这些复合材料表现为细胞生长的屏障，对标准绿脓假单胞菌和耐甲氧西林金黄色葡萄球菌菌株具有更好的生物相容性。经硅醇基团修饰的复合材料的 MTT 试验结果显示细胞存活率为 96%，表明这些样品无毒。新型合成复合材料的类型能够精细降低细胞毒性并提高 AP 的体外释放。
