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与塞尔维亚大蒜采后青霉腐烂相关的物种

Species Associated with Postharvest Blue Mold Rots of Garlic in Serbia.

作者信息

Stošić Stefan, Ristić Danijela, Trkulja Nenad, Živković Svetlana

机构信息

Department of Plant Diseases, Institute for Plant Protection and Environment, 11000 Belgrade, Serbia.

出版信息

Plant Dis. 2025 Jan;109(1):149-161. doi: 10.1094/PDIS-04-24-0890-RE. Epub 2025 Jan 15.

Abstract

Garlic ( L.) is an important plant because of its medicinal and gastronomical benefits. The host can be affected by different plant pathogens, among which species are one of the most important rot agents of stored garlic and other stored food products. This research resolves the etiology of blue mold rots associated with garlic in Serbia. -like isolates were collected (2016 to 2022) from diseased stored garlic bulbs in Serbia and identified using a polyphasic approach. Detailed characterization of the obtained cultures was performed using three solid media, three incubation temperatures, molecular and phylogenetic analyses of four molecular loci (internal transcribed spacer [ITS], beta-tubulin [], calmodulin [], and DNA-dependent RNA polymerase II second largest subunit []), and the pathogenicity assay. Five species, that is, , , , , and , were identified and confirmed as postharvest pathogens of garlic. Herein, we report for the first time the pathogenicity of and on garlic. Findings from this study provide insights into the previously unknown diversity of fungi responsible for garlic bulb decay in Serbia and will help in the assessment of the phytosanitary status of this crop, as well as the creation and application of effective disease management strategies.

摘要

大蒜(L.)因其药用和美食价值而成为一种重要的植物。宿主会受到不同植物病原体的影响,其中 物种是储存大蒜和其他储存食品最重要的腐烂病原体之一。本研究确定了塞尔维亚与大蒜相关的青霉腐烂病的病因。从塞尔维亚患病的储存大蒜鳞茎中收集了类似 的分离株(2016年至2022年),并采用多相方法进行鉴定。使用三种固体培养基、三种培养温度、对四个分子位点(内转录间隔区[ITS]、β-微管蛋白[ ]、钙调蛋白[ ]和DNA依赖性RNA聚合酶II第二大亚基[ ])进行分子和系统发育分析以及致病性测定,对获得的培养物进行了详细表征。鉴定并确认了五种 物种,即 、 、 、 和 ,它们是大蒜的采后病原体。在此,我们首次报告了 和 对大蒜的致病性。本研究结果为塞尔维亚此前未知的导致大蒜鳞茎腐烂的 真菌多样性提供了见解,并将有助于评估该作物的植物检疫状况,以及制定和应用有效的病害管理策略。

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