Lin Helen H, Lim Irene, Sletten Ellen M
Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young Dr., East Los Angeles, CA, 90095, USA.
Present Address: Department of Radiology, Stanford University, 1201 Welch Rd., Stanford, CA, 94305, USA.
Chemistry. 2024 Nov 15;30(64):e202402514. doi: 10.1002/chem.202402514. Epub 2024 Oct 16.
Fluorofluorophores are a unique class of fluorophores that can be solubilized in perfluorocarbons (PFCs) and used to study biological systems. However, because of the low dielectric constant and high oxygen solubility in the fluorous phase, the brightness and photostability of the fluorofluorophores are significantly diminished. Here, we leveraged the tight ion pairing in the fluorous phase to improve the photophysical properties of a fluorous soluble pentamethine dye (FCy5) via counterion exchange. We found that larger, softer, fluorinated, aryl borate counterions promote the ideal polymethine state where charge delocalization across the polymethine chain increases the brightness (6-fold) and photostability (55-fold) of FCy5.
氟代荧光团是一类独特的荧光团，可溶解于全氟化碳（PFCs）中并用于研究生物系统。然而，由于氟相中的低介电常数和高氧溶解度，氟代荧光团的亮度和光稳定性会显著降低。在此，我们利用氟相中的紧密离子对，通过抗衡离子交换来改善一种可溶于氟相的五甲川染料（FCy5）的光物理性质。我们发现，更大、更软的氟化芳基硼酸抗衡离子能促进理想的聚甲川状态，其中电荷在聚甲川链上的离域增加了FCy5的亮度（6倍）和光稳定性（55倍）。
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