University of Gondar Comprehensive Specialized Hospital, Gondar, Ethiopia.
Department of Hematology and Immunohematology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Science, University of Gondar, Gondar, Ethiopia.
BMC Infect Dis. 2024 Sep 4;24(1):917. doi: 10.1186/s12879-024-09811-1.
K. pneumoniae become multidrug-resistant (MDR) and commonly poses a serious health threat to patients due to limited therapeutic options. As a result, determining the prevalence and antimicrobial susceptibility patterns of K. pneumoniae isolates from clinical specimens is substantial to patient diagnosis and treatment.
A retrospective cross-sectional study was conducted from July 2021 to July 2022 at the University of Gondar Comprehensive Specialized Hospital, Northwest Ethiopia. Sociodemographic and laboratory data were collected from registered books using a data collection sheet. All types of samples were collected and processed using standard procedures. Identification of K. pneumoniae was done using Gram stain, colony characterization on culture media, anda series of biochemical tests. Antimicrobial susceptibility testing was done by the Kirby Bauer disc diffusion technique. The data were entered using Epi-info version 7 and exported to SPSS version 20 for analysis.
Among 2600 clinical specimens, 735 (28.3%) were positive for bacteria, and K. pneumoniae isolates accounted for 147 (20%). Most of them were isolated from neonates and mainly obtained from blood specimens (81.6%). These isolates were 100% resistant to Nalidixic acid, Cefotaxime, and Cefazolin. About 84% and 83.3% of the isolates were also resistant to Ceftriaxone and Tetracycline, respectively. However, they are sensitive to Nitrofurantoin (86.6%), Imipenem (85.7%), Meropenem (79%), and Amikacin (78.3%). The overall proportion of MDR K. pneumoniae isolates accounted for 57.1%.
The magnitude of MDR K. pneumoniae was very alarming. Therefore, strengthening antimicrobial stewardship programs and antimicrobial surveillance practices is strongly recommended in the study area.
由于治疗选择有限，肺炎克雷伯菌变得对多种药物耐药（MDR），并经常对患者的健康构成严重威胁。因此，确定临床标本中肺炎克雷伯菌分离株的流行率和抗菌药物敏感性模式对于患者的诊断和治疗至关重要。
这是一项在 2021 年 7 月至 2022 年 7 月在埃塞俄比亚西北部贡德尔大学综合专科医院进行的回顾性横断面研究。使用数据收集表从登记册中收集社会人口学和实验室数据。使用标准程序收集和处理所有类型的样本。使用革兰氏染色、培养基上的菌落特征和一系列生化试验来鉴定肺炎克雷伯菌。采用 Kirby Bauer 纸片扩散法进行抗菌药物敏感性试验。使用 Epi-info 版本 7 输入数据，并将其导出到 SPSS 版本 20 进行分析。
在 2600 份临床标本中，有 735 份（28.3%）为细菌阳性，肺炎克雷伯菌分离株占 147 份（20%）。它们主要分离自新生儿，主要从血液标本中获得（81.6%）。这些分离株对萘啶酸、头孢噻肟和头孢唑林的耐药率均为 100%。约 84%和 83.3%的分离株分别对头孢曲松和四环素耐药，但对呋喃妥因（86.6%）、亚胺培南（85.7%）、美罗培南（79%）和阿米卡星（78.3%）敏感。总体多药耐药肺炎克雷伯菌分离株的比例为 57.1%。
MDR 肺炎克雷伯菌的严重程度非常惊人。因此，强烈建议在研究区域加强抗菌药物管理计划和抗菌药物监测实践。
