Sierra-Mejia Andrea, Villamor Dan E V, Tzanetakis Ioannis E
Department of Entomology and Plant Pathology, Division of Agriculture, University of Arkansas System Fayetteville, AR, 72701, USA.
Department of Entomology and Plant Pathology, Division of Agriculture, University of Arkansas System Fayetteville, AR, 72701, USA.
Virus Res. 2024 Dec;350:199460. doi: 10.1016/j.virusres.2024.199460.
Blackberry chlorotic ringspot virus (BCRV) was described about 20 years ago and since then there have been several publications of the virus infecting rosaceous hosts including blackberry, raspberry, rose and apple at high rates. Still the effect of the virus on disease development is poorly understood. Aiming to bridge this knowledge gap, we developed a BCRV infectious clone and virus-induced gene silencing vector (VIGS). The infectious clone can induce systemic infection with the transmissibility of the recombinant virus evaluated through mechanical transmission. The VIGS induced silencing using two different inserts, proving the versatility of the construct. The products of this work can be used to study disease development and control as well as functional genomics studies of BCRV hosts.
黑莓褪绿环斑病毒（BCRV）约在20年前被发现，自那时起，已有多篇关于该病毒以高感染率侵染包括黑莓、树莓、玫瑰和苹果在内的蔷薇科寄主的报道。然而，人们对该病毒在病害发展过程中的作用仍知之甚少。为了填补这一知识空白，我们构建了一个BCRV感染性克隆和病毒诱导基因沉默载体（VIGS）。该感染性克隆能够引发系统感染，并通过机械接种评估重组病毒的传播能力。VIGS利用两种不同的插入片段诱导基因沉默，证明了该构建体的多功能性。本研究成果可用于研究病害发展与防治，以及BCRV寄主的功能基因组学研究。
