Evgen'ev M B, Zatsepina O G, Kakpakov V T, Vlasova I E
Mol Biol (Mosk). 1985 Mar-Apr;19(2):483-8.
The combined action of ecdysone and temperature elevation (heat shock) on D. melanogaster polytene chromosome transcription has been investigated. It was shown that heat shock while blocking the transcription in ecdysone--induced puffs is not effective in decreasing their size. Thus we are able to observe chromatin decondensation without transcription. The indirect immunofluorescence using an antiserum directed against DNA/RNA hybrids revealed an intense fluorescence in the polytene chromosomes of heat shocked larvae both in the actively transcribed heat shock puffs and in ecdysone stimulated "primary puffs" with blocked transcription. Ecdysone introduced into the media during heat shock is unable to induce any puffs. On the other hand the hormone introduced into the media after temperature elevation (30' of heat shock + 1 hour of ecdysone stimulation) induces some of the "primary puffs". A new approach for cloning the genes inducible by ecdysone and other inducible loci as well has been developed using the data obtained.
研究了蜕皮激素和温度升高（热休克）对黑腹果蝇多线染色体转录的联合作用。结果表明，热休克在阻断蜕皮激素诱导的胀泡转录时，对减小其大小无效。因此，我们能够观察到无转录的染色质解聚。使用针对DNA/RNA杂交体的抗血清进行的间接免疫荧光显示，在热休克幼虫的多线染色体中，无论是在活跃转录的热休克胀泡中还是在转录受阻的蜕皮激素刺激的“初级胀泡”中，都有强烈的荧光。在热休克期间引入培养基中的蜕皮激素不能诱导任何胀泡。另一方面，在温度升高后（热休克30分钟+蜕皮激素刺激1小时）引入培养基中的激素会诱导一些“初级胀泡”。利用所获得的数据，开发了一种克隆蜕皮激素诱导基因和其他可诱导位点的新方法。
