Lo Pei-Hsin, Huang Jin-Hsing, Chang Chia-Chih, Namisy Ahmed, Chen Chi-Yu, Chung Wen-Hsin
Section of Crop Environment, Taichung District Agricultural Research and Extension Station, Ministry of Agriculture, Changhua 515008, Taiwan.
Department of Plant Pathology, National Chung Hsing University, Taichung 402202, Taiwan.
Plant Dis. 2025 Jan;109(1):170-182. doi: 10.1094/PDIS-06-24-1161-RE. Epub 2025 Jan 17.
species complex (FSSC) is a causal agent of collar rot and fruit rot in passion fruit worldwide. This study investigated the diversity and characteristics of FSSC isolates causing collar rot and fruit rot in Taiwanese passion fruit. Thirty-five FSSC isolates were harvested from collar rot and fruit rot samples of passion fruit from various cultivars and different geographical locations in Taiwan. The majority of these FSSC isolates caused collar rot and fruit rot disease of varying virulence in the stems and fruits of the purple and yellow cultivars of passion fruit. FSSC isolates were categorized into four groups: . - (FSSC 21; = 29), . (FSSC 5; = 1), . (FSSC 24; = 1), and an unknown group ( = 4) based on the phylogenetic analysis of internal transcribed sequence (ITS), translation elongation factor 1 alpha (TEF-1α), and RNA polymerase II subunit 2 (RPB2) sequences. In Taiwan, . - was the dominant species causing collar rot and fruit rot in passion fruit. . - was a homothallic fungus that produced perithecia in diseased tissues. However, and . did not produce perithecia. The unknown FSSC group showed morphological characteristics similar to . - and produced perithecia. Phylogenetic analysis based on the ITS and TEF-1α sequences demonstrated that the Taiwanese FSSC isolates were distinct from the Brazilian and Chinese FSSC isolates. In summary, FSSC isolates causing collar rot and fruit rot of Taiwanese passion fruit showed high diversity, potentially associated with the geographical locations.
镰刀菌复合种（FSSC）是全球百香果颈腐病和果腐病的病原菌。本研究调查了引起台湾百香果颈腐病和果腐病的FSSC分离株的多样性和特征。从台湾不同品种和不同地理位置的百香果颈腐病和果腐病样本中采集了35株FSSC分离株。这些FSSC分离株中的大多数在紫色和黄色品种百香果的茎和果实中引起了不同毒力的颈腐病和果腐病。根据内部转录间隔区（ITS）、翻译延伸因子1α（TEF-1α）和RNA聚合酶II亚基2（RPB2）序列的系统发育分析，FSSC分离株被分为四组：. - （FSSC 21；n = 29），. （FSSC 5；n = 1），. （FSSC 24；n = 1），以及一个未知组（n = 4）。在台湾，. - 是引起百香果颈腐病和果腐病的优势种。. - 是一种同宗配合真菌，在患病组织中产生子囊壳。然而，. 和. 不产生子囊壳。未知的FSSC组显示出与. - 相似的形态特征并产生子囊壳。基于ITS和TEF-1α序列的系统发育分析表明，台湾的FSSC分离株与巴西和中国的FSSC分离株不同。总之，引起台湾百香果颈腐病和果腐病的FSSC分离株表现出高度的多样性，可能与地理位置有关。
