蛋白质组学和数字减影血管造影方法显示 CDH18 是治疗烟雾病的潜在靶点。
Proteomics and digital subtraction angiography approaches reveal CDH18 as a potential target for therapy of moyamoya disease.
机构信息
Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, 450052, China.
出版信息
Biol Direct. 2024 Sep 5;19(1):76. doi: 10.1186/s13062-024-00522-w.
Moyamoya disease, characterized by basal cerebral artery obstruction, was studied for differential protein expression to elucidate its pathogenesis. Proteomic analysis of cerebrospinal fluid from 10 patients, categorized by postoperative angiography into good and poor prognosis groups, revealed 46 differentially expressed proteins. Notably, cadherin 18 (CDH18) was the most significantly upregulated in the good prognosis group. In addition, the expression of cadherin 18 (CDH18) and phenotypic transformation-related proteins were measured by qRT-PCR and western blot. The effects of CDH18 in vascular smooth muscle cells were detected by CCK-8, EdU, transwell and wound healing assays. The overexpression of CDH18 in vascular smooth muscle cells (VSMCs) was found to inhibit proliferation, migration, and phenotypic transformation. These findings suggest CDH18 as a potential therapeutic target in moyamoya disease.
烟雾病，特征为基底动脉阻塞，研究其差异蛋白质表达以阐明其发病机制。对 10 例患者的脑脊液进行蛋白质组学分析，根据术后血管造影分为预后良好和预后不良组，发现 46 种差异表达蛋白。值得注意的是，钙黏蛋白 18（CDH18）在预后良好组中表达上调最为显著。此外，通过 qRT-PCR 和 Western blot 检测钙黏蛋白 18（CDH18）和表型转化相关蛋白的表达。通过 CCK-8、EdU、transwell 和划痕愈合实验检测 CDH18 在血管平滑肌细胞中的作用。发现血管平滑肌细胞（VSMCs）中 CDH18 的过表达可抑制增殖、迁移和表型转化。这些发现提示 CDH18 可能成为烟雾病的潜在治疗靶点。
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