Cho S, Scharpf S, Franko M, Vermeulen C W
Biochem Biophys Res Commun. 1985 May 16;128(3):1268-73. doi: 10.1016/0006-291x(85)91077-0.
In steady state E. coli cells growing at their maximal rate in broth, maximum induction of beta-galactosidase occurs at 0.10 mM isopropyl-thio-beta-D-galactoside (IPTG). Although induction of lac is near zero in steady state cells that are growing in 0.01 mM IPTG, induction at mildly subdued levels persists down to at least 0.001 mM in post-steady state cultures. Meanwhile, thiogalactoside transacetylase remains uninduced over the full range in which the cells are in steady state.
在肉汤中以最大速率生长的稳态大肠杆菌细胞中,β-半乳糖苷酶的最大诱导发生在0.10 mM异丙基硫代-β-D-半乳糖苷(IPTG)时。尽管在0.01 mM IPTG中生长的稳态细胞中乳糖操纵子的诱导接近零,但在稳态后培养物中,低至至少0.001 mM时仍存在轻度抑制水平的诱导。同时,硫代半乳糖苷转乙酰酶在细胞处于稳态的整个范围内都未被诱导。