College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin 150030, PR China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin 150030, PR China.
Int J Biol Macromol. 2024 Nov;279(Pt 3):135360. doi: 10.1016/j.ijbiomac.2024.135360. Epub 2024 Sep 5.
The multi-drug resistance of methicillin-resistant Staphylococcus aureus (MRSA) and complex wound microenvironment challenge the repair of MRSA infected wound. Herein, in this study, α-tocopherol modified glycol chitosan (TG) nanoparticles encapsulated with phytochemical rhein (Rhein@TG NPs) were prepared for comprehensive anti-infection and promotion of MRSA infected wound healing. Rhein@TG NPs could not only specifically release rhein in the infection site in response to low pH and lipase of infectious microenvironment, but also up-regulated M1 macrophage polarization in the infection stage, thus achieving synergistically bacterial elimination with low possibility of developing resistance. Additionally, the NPs reduced the levels of pro-inflammatory factors in the post-infection stage, scavenged the ROS, promoted cell migration and angiogenesis, which significantly improved the microenvironment of infected wound healing. Therefore, this antibiotic-free NPs enabling anti-infection and promotion of wound healing provides a new and long-term strategy for the treatment of MRSA infected wound.
耐甲氧西林金黄色葡萄球菌（MRSA）的多药耐药性和复杂的伤口微环境挑战了 MRSA 感染伤口的修复。在此，在本研究中，制备了用植物化学物质大黄酸（Rhein）包封的 α-生育酚改性的壳聚糖（TG）纳米颗粒（Rhein@TG NPs），用于全面抗感染和促进 MRSA 感染性伤口愈合。Rhein@TG NPs 不仅可以在感染部位特异性释放响应低 pH 值和感染微环境中的脂肪酶的大黄酸，而且还可以在上感染阶段上调 M1 巨噬细胞极化，从而以低耐药性的可能性实现协同细菌消除。此外，这些纳米颗粒降低了感染后阶段促炎因子的水平，清除了 ROS，促进了细胞迁移和血管生成，显著改善了感染伤口愈合的微环境。因此，这种无抗生素纳米颗粒实现抗感染和促进伤口愈合为治疗 MRSA 感染性伤口提供了一种新的、长期的策略。
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