State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research Institute, CAAS, 678 Haping Road, Harbin, Heilongjiang 150069, China; College of Life Science and Engineering, Foshan University, 33 Guangyun Road, Foshan, Guangdong 528231, China.
State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research Institute, CAAS, 678 Haping Road, Harbin, Heilongjiang 150069, China.
Vet Microbiol. 2024 Nov;298:110239. doi: 10.1016/j.vetmic.2024.110239. Epub 2024 Sep 3.
African swine fever (ASF), a highly infectious and devastating disease affecting both domestic pigs and wild boars, owes its etiology to African swine fever virus (ASFV). ASFV encodes more than 165 proteins. However, novel immunogenic proteins remain unknown. This study aimed to determine the antigenicity of the F317L protein (pF317L) of ASFV. The results revealed that pF317L was able to react with convalescent pig sera, indicating that pF317L could be a candidate antigen. The antigenic potential of pF317L expressed by rHCLV-F317L, a recombinant virus in the backbone of C-strain (a lapinized live attenuated classical swine fever virus) was further investigated in rabbits and pigs. The results revealed that antibodies and cell-mediated immune responses against pF317L were induced in either rabbits or pigs inoculated with rHCLV-F317L. Importantly, anti-pF317L antibodies from rabbits or pigs immunized with rHCLV-F317L significantly inhibited ASFV replication in vitro. In conclusion, pF317L demonstrates favorable immunogenic properties, positioning it as a promising candidate for the development of protective antigens in the ongoing endeavor to formulate efficacious ASF vaccine strategies.
非洲猪瘟（ASF）是一种高度传染性和毁灭性的疾病，影响家猪和野猪，其病因是非洲猪瘟病毒（ASFV）。ASFV 编码超过 165 种蛋白质，但新型免疫原性蛋白质仍未知。本研究旨在确定 ASFV 的 F317L 蛋白（pF317L）的抗原性。结果表明，pF317L 能够与康复猪血清发生反应，表明 pF317L 可能是候选抗原。在 C 株（兔化弱毒经典猪瘟病毒）骨架上表达的重组病毒 rHCLV-F317L 表达的 pF317L 的抗原潜力在兔和猪中进一步进行了研究。结果表明，接种 rHCLV-F317L 的兔或猪均诱导了针对 pF317L 的抗体和细胞介导的免疫反应。重要的是，用 rHCLV-F317L 免疫的兔或猪的抗-pF317L 抗体可显著抑制体外的 ASFV 复制。总之，pF317L 具有良好的免疫原性，为开发有效的 ASF 疫苗策略中的保护性抗原提供了有希望的候选物。
Front Cell Infect Microbiol. 2024-12-20
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