Bisaillon J G, Lafond L, Beaudet R, Saheb S A, Sylvestre M
Exp Biol. 1985;43(4):231-42.
Coagulase-negative staphylococci are known to produce bactericidal and bacteriostatic antigonococcal activities. Out of 12 staphylococci, Staphylococcus haemolyticus (four isolates) was identified as the principal source of bactericidal activity, whereas Staphylococcus epidermidis (six isolates) was primarily responsible for producing bacteriostatic activity. A comparison of the bacteriostatic substance produced by S. epidermidis isolate 66 with the bactericidal substance produced by S. haemolyticus isolate 7, which had been previously purified, showed that they were similar lipoproteins or lipid-associated proteins. However, these two inhibitors migrated differently under electrophoresis on agarose gel. The protein component of the bacteriostatic inhibitor was more difficult to separate from the lipid component when chromatographed on Ultrogel AcA 54 in the presence of urea (4 M) than the protein component of the bactericidal inhibitor. The protein component of both types of inhibitors was responsible for the antigonococcal activity and was dissociable into subunits of approximately 1,400 daltons. However, these protein components had different migration patterns on agarose gel. The bacteriostatic substance displayed a bactericidal effect when dissociated from its lipidic component suggesting that the lipids might play a role in the type of inhibitory activity produced. All of the bactericidal and bacteriostatic substances contained in the different crude preparations were antigenically related.
已知凝固酶阴性葡萄球菌可产生抗淋球菌的杀菌和抑菌活性。在12株葡萄球菌中，溶血葡萄球菌（4株分离株）被鉴定为杀菌活性的主要来源，而表皮葡萄球菌（6株分离株）主要负责产生抑菌活性。将表皮葡萄球菌分离株66产生的抑菌物质与先前已纯化的溶血葡萄球菌分离株7产生的杀菌物质进行比较，结果表明它们是相似的脂蛋白或脂质相关蛋白。然而，这两种抑制剂在琼脂糖凝胶电泳中的迁移情况不同。当在4M尿素存在下于Ultrogel AcA 54上进行层析时，抑菌抑制剂的蛋白质成分比杀菌抑制剂的蛋白质成分更难与脂质成分分离。两种类型抑制剂的蛋白质成分均具有抗淋球菌活性，且可解离成约1400道尔顿的亚基。然而，这些蛋白质成分在琼脂糖凝胶上具有不同的迁移模式。抑菌物质从其脂质成分解离后显示出杀菌作用，这表明脂质可能在产生的抑制活性类型中起作用。不同粗制品中所含的所有杀菌和抑菌物质在抗原性上相关。
