Graduate School of Integrated Science and Technology, Shizuoka University, Shizuoka 422-8529, Japan.
College of Agriculture, Academic Institute, Shizuoka University, Shizuoka 422-8529, Japan.
ACS Chem Biol. 2024 Sep 20;19(9):1896-1903. doi: 10.1021/acschembio.4c00266. Epub 2024 Sep 9.
The class III lanthipeptide synthetase (LanKC) installs unusual amino acids, such as lanthionine and labionin, in lanthipeptides. Through genome mining, we discovered a new class III lanthipeptide synthetase coding gene () and precursor peptide coding genes (, , and ) in the genome of the actinobacterium . Coexpression experiments of the biosynthetic genes in resulted in the production of new lanthipeptides named nocardiopeptins A1-A3. Analysis of two-dimensional NMR spectra after enzymatic degradation and partial basic hydrolysis of nocardiopeptin A2 revealed that labionin was located in lanthionine with opposite orientations, forming a nesting structure in nocardiopeptin A2. To the best of our knowledge, this bridging pattern in the lanthipeptides was unprecedented, indicating a novel reaction characteristic of the class III lanthipeptide synthetase NptKC.
III 类硫肽合成酶（LanKC）在硫肽中安装不寻常的氨基酸，如硫氨酸和拉宾宁。通过基因组挖掘，我们在放线菌的基因组中发现了一个新的 III 类硫肽合成酶编码基因（）和前体肽编码基因（、、和）。在中表达生物合成基因的共表达实验导致了新的硫肽nocardiopeptins A1-A3 的产生。nocardiopeptin A2 的酶解和部分碱性水解后的二维 NMR 谱分析表明，拉宾宁位于硫氨酸的相反方向，在 nocardiopeptin A2 中形成嵌套结构。据我们所知，这种硫肽中的桥接模式是前所未有的，表明 III 类硫肽合成酶 NptKC 的新反应特征。
