Taghizade Tayebe, Akbarzadeh-Baghban Alireza, Navab Safa Nasrin
Department of Audiology, Faculty of Rehabilitation, Tehran University of Medical Sciences, Tehran, Iran.
Proteomics Research Center, Department of Biostatistics, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AMB Express. 2024 Sep 11;14(1):101. doi: 10.1186/s13568-024-01748-7.
In this paper we investigate the influence of cold plasma as novel method on the external otitis treatment which is a frequent cause of earache. 24 infected external auditory canals in 24 rats were categorized in four experimental groups including control, plasma exposed, ciprofloxacin drug and mixed of plasma-ciprofloxacin groups. In plasma group, dielectric barrier discharge was employed as the source of cold plasma in 5 days. All rats were observed with otoscope daily and a scoring system was used to evaluate swelling and effusion of the ear canal. Number of colonies in microbiological culture were counted in each group during the first 5 days after treatment. For the multiple group comparisons of swelling and effusion measured in the external auditory canal, Kruskal-Wallis analysis was applied and one-way anova and Kruskal-Wallis analysis was used for the statistical analysis of the results of the cultures in different days. Also, Tukey and Mann-Whitney tests was applied for multiple comparisons. Our findings show that swelling and effusion were obviously reduced in plasma group compared to control group (P < 0.01). Number of colonies in control group was statistically different from those in drug, plasma, and mixed group on the second to fifth day (p < 0.001). According to the results cold plasma can be introduced as an impressive method for external otitis treatment. Moreover, when cold plasma joined to antibiotic method, it leads to a superior performance respecting plasma or antibiotic method alone.
在本文中，我们研究了冷等离子体作为一种新方法对外耳道炎治疗的影响，外耳道炎是耳痛的常见原因。将24只大鼠的24个受感染外耳道分为四个实验组，包括对照组、等离子体暴露组、环丙沙星药物组和等离子体-环丙沙星混合组。在等离子体组中，采用介质阻挡放电作为冷等离子体源，持续5天。每天用耳镜观察所有大鼠，并使用评分系统评估耳道的肿胀和积液情况。在治疗后的前5天，对每组微生物培养中的菌落数进行计数。对于外耳道测量的肿胀和积液的多组比较，应用Kruskal-Wallis分析，对于不同天数培养结果的统计分析，使用单因素方差分析和Kruskal-Wallis分析。此外，使用Tukey和Mann-Whitney检验进行多重比较。我们的研究结果表明，与对照组相比，等离子体组的肿胀和积液明显减少（P < 0.01）。在第2天至第5天，对照组的菌落数与药物组、等离子体组和混合组的菌落数在统计学上有差异（p < 0.001）。根据结果，冷等离子体可作为一种有效的外耳道炎治疗方法引入。此外，当冷等离子体与抗生素方法联合使用时，相对于单独使用等离子体或抗生素方法，其表现更优。
