蜂王浆及其功能成分对 MKN-28 胃癌细胞增殖的抑制作用。

Inhibitory Effects of Royal Jelly and Its Functional Components on the Proliferation of MKN-28 Gastric Cancer Cells.

机构信息

College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, China.

Jiangsu Lishui Huiyuan Nutritional Health Products Co., Nanjing 211215, China.

出版信息

J Agric Food Chem. 2024 Sep 25;72(38):20918-20929. doi: 10.1021/acs.jafc.4c03367. Epub 2024 Sep 12.

DOI:10.1021/acs.jafc.4c03367

PMID:39262347

Abstract

Royal jelly (RJ) is a natural food product with nutritional value and anticancer activity. However, their effects on gastric cancer are unclear. Here, we show that treatment with 5-320 μg/mL of RJ, ethanol extract (RJEE), and protein hydrolyzate (RJPH) decreased the viability of MKN-28 gastric cancer cells, with a half-maximal inhibitory concentration of 123.22 μg/mL for RJEE. RJ, RJEE, and RJPH increase the lactate dehydrogenase release rate and change the morphology of the cells, resulting in cell shrinkage, nucleoplasm condensation, and the formation of apoptotic bodies. RJ and its functional components stagnated the cell cycle in the G0/G1 phase, accompanied by the accumulation of reactive oxygen species, decreased mitochondrial membrane potential, and increased expression levels of p53 and p21 proteins, caspase-3 activation, and apoptosis. Therefore, RJ, RJEE, and RJPH have potential inhibitory effects on the proliferation of gastric cancer cells.

摘要

蜂王浆（RJ）是一种具有营养价值和抗癌活性的天然食品。然而，它们对胃癌的影响尚不清楚。在这里，我们表明，用 5-320 μg/mL 的 RJ、乙醇提取物（RJEE）和蛋白质水解物（RJPH）处理可降低 MKN-28 胃癌细胞的活力，RJEE 的半最大抑制浓度为 123.22 μg/mL。RJ、RJEE 和 RJPH 增加乳酸脱氢酶释放率并改变细胞形态，导致细胞收缩、核质浓缩和凋亡小体形成。RJ 及其功能成分使细胞周期停滞在 G0/G1 期，同时伴随着活性氧的积累、线粒体膜电位降低以及 p53 和 p21 蛋白表达水平升高、caspase-3 激活和细胞凋亡。因此，RJ、RJEE 和 RJPH 对胃癌细胞的增殖具有潜在的抑制作用。