College of Chemistry and Materials Engineering, Institute of Ocean, Bohai University, Jinzhou, 121013, China.
College of Food Science and Technology, Bohai University, Food Safety Key Lab of Liaoning Province, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, 121013, China.
Anal Chim Acta. 2024 Oct 23;1327:343163. doi: 10.1016/j.aca.2024.343163. Epub 2024 Aug 28.
Hydrazine (NH) serves as a crucial industrial raw material and finds extensive applications in the fields of medicine, pesticides, ecological environment, and textile dyes. Excessive residue of hydrazine will cause significant toxicity risks to the ecosystem and human health. Traditional detection methods often require multi-step pretreatment of samples, and complex instrumentation, and are time-consuming, which is not conducive to rapid on-site detection. Therefore, it is imperative to develop a method suitable for rapid detection of NH in multiple fields.
In this study, we constructed a red emission fluorescent probe (BCM). BCM can recognize NH by colorimetric and fluorescence dual-channel response with a good anti-interference ability and a low detection limitation. The fluorescence emission of BCM is attributed to the ICT effect by DFT calculations, and a new product 3H-benzo[f]chromene-2-carbaldehyde hydrazine is formed after BCM recognition of NH. A linear relationship was established between the ratio of red-blue (R/B) coming from the fluorescence color of BCM and the NH level. Hence, a BCM-based smartphone sensing platform for detecting NH was developed, and the NH content can be rapidly detected with satisfactory accuracy in the lake water samples. In addition, the residues of NH in soils, plants and food samples can be visualized, and BCM can image for NH in living cells, as well as NH vapor can be detected by using the electrospinning film loaded with BCM.
In particular, the fluorescent probe BCM can be combined with a smartphone for the detection and visual imaging of hydrazine in environmental samples. We believe the BCM and smartphone-based sensing platforms constructed in this paper will be a powerful tool for visual quantitative detection of NH in the fields of food safety assessment, bioimaging, and environmental protection.
肼（NH）作为一种重要的工业原料，广泛应用于医学、农药、生态环境和纺织染料等领域。肼的过量残留会对生态系统和人类健康造成重大毒性风险。传统的检测方法通常需要对样品进行多步预处理，且仪器复杂，耗时较长，不利于快速现场检测。因此，开发一种适用于多个领域快速检测 NH 的方法迫在眉睫。
本研究构建了一种红色发射荧光探针（BCM）。BCM 可以通过比色和荧光双通道响应识别 NH，具有良好的抗干扰能力和较低的检测极限。BCM 的荧光发射归因于 DFT 计算的 ICT 效应，并且在 BCM 识别 NH 后形成新产物 3H-苯并[f]色烯-2-醛腙。BCM 的荧光颜色的红-蓝（R/B）比值与 NH 水平之间建立了线性关系。因此，开发了基于 BCM 的智能手机传感平台用于检测 NH，可以在湖水样品中快速检测到 NH，并且具有令人满意的准确性。此外，还可以可视化土壤、植物和食物样品中的 NH 残留，并且可以使用负载 BCM 的静电纺丝膜来对活细胞内的 NH 进行成像，以及检测 NH 蒸气。
特别是，荧光探针 BCM 可以与智能手机结合，用于检测和可视化环境样品中的肼。我们相信，本文构建的 BCM 和基于智能手机的传感平台将成为食品安全评估、生物成像和环境保护等领域中用于 NH 可视化定量检测的有力工具。
