Kaur Prabhjot, Sharma Jitender, Bhardwaj Nishi Kant, Bhardwaj Shubhang, Kaur Daljeet, Singh Amarjit, Kumar Ashish
Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119 Haryana India.
Avantha Centre for Industrial Research and Development, Paper Mill Campus, Yamuna Nagar, 135001 Haryana India.
3 Biotech. 2024 Oct;14(10):228. doi: 10.1007/s13205-024-04080-w. Epub 2024 Sep 10.
High-purity cellulose from paper pulp can be obtained after appropriate treatments involving pure xylanases and cellulases/endoglucanases. This study investigated the efficacy of using crude xylanase and cellulase instead of commercial ones to improve process economics. Kraft paper grade pulp produced from veneer waste, hardwood, and non-wood sources was utilized as a more sustainable option. Crude xylanase and cellulase from isolated soil bacteria 3GAH and PJK6 were used for process optimization. The correlation between Fock reactivity, chain scission, and crystallinity after crude-cellulase treatment was established through chemical, FTIR, and XRD analyses. Pentosans in kraft pulp were reduced from an initial 18.7% to 4.9% through sequential treatments with crude xylanase and alkali. Subsequent crude-cellulase treatment, even at 8 U/g o.d. pulp, improved Fock reactivity from 28.2% to 61.2%, fulfilling a major criterion for viscose. Thus, crude enzymes can be effectively used for the efficient and economical upgrading of paper pulp to dissolving pulp.
经过涉及纯木聚糖酶和纤维素酶/内切葡聚糖酶的适当处理后，可从纸浆中获得高纯度纤维素。本研究调查了使用粗木聚糖酶和纤维素酶替代商业酶以提高工艺经济性的效果。利用单板废料、硬木和非木材来源生产的牛皮纸级纸浆作为更具可持续性的选择。使用从分离的土壤细菌3GAH和PJK6中提取的粗木聚糖酶和纤维素酶进行工艺优化。通过化学分析、傅里叶变换红外光谱（FTIR）分析和X射线衍射（XRD）分析，确定了粗纤维素酶处理后福克反应性、断链和结晶度之间的相关性。通过用粗木聚糖酶和碱进行顺序处理，牛皮纸浆中的戊聚糖含量从最初的18.7%降至4.9%。随后的粗纤维素酶处理，即使在8 U/g绝干纸浆的用量下，也将福克反应性从28.2%提高到61.2%，满足了粘胶纤维的一个主要标准。因此，粗酶可有效地用于将纸浆高效经济地升级为溶解浆。
