Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, China National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2024 Aug 30;25(17):9456. doi: 10.3390/ijms25179456.
Oriental hybrid lilies, known for their vibrant colors, diverse flower shapes, and long blooming seasons, require annual bulb propagation in horticultural production. This necessity can lead to higher production costs and limit their use in landscaping. The LA hybrid lily 'Aladdin' has shown strong self-reproduction capabilities in optimal cultivation environments, producing numerous high-quality underground stem bulblets. This makes it a valuable model for studying bulblet formation in lilies under natural conditions. Through transcriptome data analysis of different developmental stages of 'Aladdin' bulblets, the gene, linked to bulblet formation, was identified. Bioinformatics analysis, subcellular localization studies, and transcriptional activation activity tests were conducted to understand the characteristics of . By introducing the gene into 'Sorbonne' aseptic seedlings via Agrobacterium-mediated transformation, resistant plants were obtained. Positive plants were identified through various methods such as GUS activity detection, PCR, and fluorescence quantitative PCR. Phenotypic changes in positive plants were observed, and various physiological indicators were measured to confirm the role of in bulblet formation, including soluble sugar content, starch content, sucrose synthase activity, and endogenous hormone levels. The findings suggest that the gene plays a significant role in promoting the development of lily bulblets, offering insights for enhancing the reproductive capacity of Oriental hybrid lilies and exploring the molecular mechanisms involved in lily bulb regeneration.
东方杂种百合以其鲜艳的色彩、多样的花朵形状和长花期而闻名，在园艺生产中需要每年进行鳞茎繁殖。这种必要性导致生产成本更高，并限制了它们在景观美化中的应用。LA 杂种百合 'Aladdin' 在最佳栽培环境下表现出强大的自我繁殖能力，产生了许多高质量的地下茎鳞茎。这使其成为研究百合在自然条件下鳞茎形成的有价值模型。通过对 'Aladdin' 鳞茎不同发育阶段的转录组数据分析，鉴定出与鳞茎形成相关的 基因。通过生物信息学分析、亚细胞定位研究和转录激活活性测试，研究了 的特征。通过农杆菌介导的转化将 基因导入 'Sorbonne' 无菌幼苗中，获得了抗性植物。通过 GUS 活性检测、PCR 和荧光定量 PCR 等多种方法鉴定阳性植物。观察阳性植物的表型变化，并测量各种生理指标，以确认 基因在鳞茎形成中的作用，包括可溶性糖含量、淀粉含量、蔗糖合酶活性和内源激素水平。研究结果表明， 基因在促进百合鳞茎发育中起着重要作用，为增强东方杂种百合的繁殖能力和探索百合鳞茎再生的分子机制提供了依据。
