Songsaeng Apisit, Boonchuen Pakpoom, Nareephot Phongkeat, Piromyou Pongdet, Wongdee Jenjira, Greetatorn Teerana, Inthaisong Sukanya, Tantasawat Piyada Alisha, Teamtisong Kamonluck, Tittabutr Panlada, Sato Shusei, Boonkerd Nantakorn, Songwattana Pongpan, Teaumroong Neung
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Institute of Research and Development, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Plants (Basel). 2024 Sep 5;13(17):2495. doi: 10.3390/plants13172495.
Mung bean ( L.), a vital legume in Asia with significant nutritional benefits, is highly susceptible to Cercospora leaf spot (CLS) caused by , leading to significant yield losses. As an alternative to chemical fungicides, bio-priming with rhizobacteria can enhance plant resistance. This study explores the potential of sp. strain DOA9 to augment resistance in mung bean against CLS via root priming. The results reveal that short (3 days) and double (17 and 3 days) priming with DOA9 before fungal infection considerably reduces lesion size on infected leaves by activating defense-related genes, including , , , , , , , and , or by suppressing the inhibition of and enhancing peroxidase (POD) activity in leaves. Interestingly, the Type 3 secretion system (T3SS) of DOA9 may play a role in establishing resistance in CN72. These findings suggest that DOA9 primes CN72's defense mechanisms, offering an effective bio-priming strategy to alleviate CLS. Hence, our insights propose the potential use of DOA9 as a bio-priming agent to manage CLS in CN72, providing a sustainable alternative to chemical fungicide applications.
绿豆（Vigna radiata (L.)）是亚洲一种重要的豆类，具有显著的营养价值，但极易受到由[病原菌名称缺失]引起的尾孢叶斑病（CLS）影响，导致产量大幅损失。作为化学杀菌剂的替代方法，用根际细菌进行生物引发可以增强植物抗性。本研究探讨了[菌株名称缺失] sp. 菌株DOA9通过根部引发增强绿豆对CLS抗性的潜力。结果表明，在真菌感染前用DOA9进行短期（3天）和双重（17天和3天）引发，通过激活包括[基因名称缺失]、[基因名称缺失]、[基因名称缺失]、[基因名称缺失]、[基因名称缺失]、[基因名称缺失]、[基因名称缺失]和[基因名称缺失]等与防御相关的基因，或通过抑制[物质名称缺失]的抑制作用并增强叶片中的过氧化物酶（POD）活性，可显著减小受感染叶片上的病斑大小。有趣的是，DOA9的III型分泌系统（T3SS）可能在建立绿豆CN72的抗性中发挥作用。这些发现表明，DOA9引发了绿豆CN72的防御机制，提供了一种有效的生物引发策略来减轻CLS。因此，我们的研究结果提出了将DOA9用作生物引发剂来管理绿豆CN72中的CLS的潜力，为化学杀菌剂应用提供了一种可持续的替代方法。
