Ziółkowska Dorota, Shyichuk Alexander, Shyychuk Iryna
Faculty of Chemical Technology and Engineering, Bydgoszcz University of Science and Technology, Seminaryjna 3, 85-326 Bydgoszcz, Poland.
Molecules. 2024 Aug 25;29(17):4017. doi: 10.3390/molecules29174017.
The ionic association of Alcian Blue dye with poly(styrene sulfonate) in aqueous solutions was studied for analytical purposes. The quadruple-charged cationic dye, Alcian Blue, was found to form colloidal ionic associates with poly(styrene sulfonate) anions. When the amounts of opposite charges are nearly equal, the resulting ionic associates lose solubility and coagulate rapidly. This effect occurs within a narrow range of the ratio of poly(styrene sulfonate) to Alcian Blue. At the point of charge equivalence, the zeta potential of the resulting particles is zero, which facilitates flocculation. The resulting flocs enlarge to approximately 0.05-0.5 mm and precipitate rapidly. FTIR spectroscopy confirms that the precipitate contains both poly(styrene sulfonate) and Alcian Blue dye. Sedimentation kinetics was studied in detail using scanning turbidimetry. Due to the high molar absorbance of the Alcian Blue dye at 600 nm, the point of equimolar charge ratio was precisely determined by spectrophotometry. The complete precipitation of ionic associates occurs when the amount of poly(styrene sulfonate) ranges from 1.4 to 1.55 mmol per 1 g of Alcian Blue dye. Such a narrow coagulation range allows for the use of the studied effect for quantitative analysis. Both Alcian Blue dye and poly(styrene sulfonate) can be quantified if one of their concentrations is known.
为了分析目的，研究了水溶液中阿尔新蓝染料与聚苯乙烯磺酸盐的离子缔合。发现四价阳离子染料阿尔新蓝与聚苯乙烯磺酸盐阴离子形成胶体离子缔合物。当相反电荷的量几乎相等时，生成的离子缔合物失去溶解性并迅速凝聚。这种效应在聚苯乙烯磺酸盐与阿尔新蓝的比例的狭窄范围内发生。在电荷等效点，生成颗粒的ζ电位为零，这有利于絮凝。生成的絮凝物增大到约0.05 - 0.5毫米并迅速沉淀。傅里叶变换红外光谱证实沉淀物同时含有聚苯乙烯磺酸盐和阿尔新蓝染料。使用扫描比浊法详细研究了沉降动力学。由于阿尔新蓝染料在600纳米处的高摩尔吸光度，通过分光光度法精确测定了等摩尔电荷比点。当聚苯乙烯磺酸盐的量为每1克阿尔新蓝染料1.4至1.55毫摩尔时，离子缔合物会完全沉淀。如此狭窄的凝聚范围使得可以利用所研究的效应进行定量分析。如果已知阿尔新蓝染料和聚苯乙烯磺酸盐其中一种的浓度，那么两者都可以被定量。
