Key Laboratory of Molecular Animal Nutrition of the Ministry of Education, Institute of Animal Nutrition and Feed Sciences, College of Animal Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, China.
Guizhou Light Industry Technical College, Guiyang 550025, Guizhou Province, China.
Food Res Int. 2024 Nov;195:114950. doi: 10.1016/j.foodres.2024.114950. Epub 2024 Aug 22.
Rosa roxburghii Tratt (RRT), a traditional Chinese plant known as the 'King of Vitamin C (VitC; ascorbic acid, AsA)', contains a wealth of nutrients and functional components, including polysaccharides, organic acids, flavonoids, triterpenes, and high superoxide dismutase (SOD) activity. The various functional components of RRT suggest that it may theoretically have a stronger potential for alleviating colitis compared to VitC. This study aims to verify whether RRT has a stronger ability to alleviate colitis than equimolar doses of VitC and to explore the mechanisms underlying this improvement. Results showed that RRT significantly mitigated body weight loss, intestinal damage, elevated inflammation levels, and compromised barriers in mice induced by Dextran sulfate sodium (DSS). Additionally, RRT enhanced the diversity and composition of intestinal microbiota in these DSS-induced mice. Colon RNA sequencing analysis revealed that compared to VitC, RRT further downregulated multiple immune-related signaling pathways, particularly the B cell receptor (BCR) pathway, which is centered around genes like Btk and its downstream PI3K-AKT, NF-κB, and MAPK signaling pathways. Correlation analysis between microbiota and genes demonstrated a significant relationship between the taxa improved by RRT and the key genes in the BCR and its downstream signaling pathways. Overall, RRT exhibited superior capabilities in alleviating DSS-induced colitis compared to VitC by decreasing intestinal inflammation and modulating BCR and its downstream signaling pathways, potentially regulated by the improved intestinal microbiota.
刺梨（Rosa roxburghii Tratt，RRT）是一种传统的中国植物，被誉为“维生素 C 之王（VitC；抗坏血酸，AsA）”，它含有丰富的营养物质和功能成分，包括多糖、有机酸、类黄酮、三萜和高超氧化物歧化酶（SOD）活性。RRT 的各种功能成分表明，它在理论上可能比 VitC 具有更强的缓解结肠炎的潜力。本研究旨在验证 RRT 是否比等摩尔剂量的 VitC 具有更强的缓解结肠炎的能力，并探讨其改善作用的机制。结果表明，RRT 显著减轻了葡聚糖硫酸钠（DSS）诱导的小鼠体重减轻、肠道损伤、炎症水平升高和屏障受损。此外，RRT 增强了这些 DSS 诱导的小鼠肠道微生物群的多样性和组成。结肠 RNA 测序分析表明，与 VitC 相比，RRT 进一步下调了多个免疫相关信号通路，特别是 B 细胞受体（BCR）通路，该通路围绕 Btk 及其下游 PI3K-AKT、NF-κB 和 MAPK 信号通路等基因。微生物群和基因之间的相关性分析表明，RRT 改善的分类群与 BCR 及其下游信号通路中的关键基因之间存在显著关系。总体而言，RRT 通过降低肠道炎症和调节 BCR 及其下游信号通路，在缓解 DSS 诱导的结肠炎方面表现出优于 VitC 的能力，这可能受改善的肠道微生物群调节。
