Cochran F R, Connor J R, Roddick V L, Waite B M
Biochem Biophys Res Commun. 1985 Jul 31;130(2):800-6. doi: 10.1016/0006-291x(85)90487-5.
To identify the source of arachidonic acid utilized for eicosanoid production, rabbit alveolar macrophages were challenged with 1.0 microM 12-O-tetradecanoylphorbol-13-acetate (TPA) or 3.8 microM Ca+2 ionophore A23187 for 3 h. Upon stimulation with TPA, a loss of [3H]arachidonic acid from phosphatidylcholine, phosphatidylethanolamine, lyso(bis)phosphatidic acid, and phosphatidylserine/phosphatidylinositol was observed. Although calcium ionophore stimulated the liberation of arachidonate solely from phosphatidyl-ethanolamine and phosphatidylcholine, it proved to be a poor stimulus for macrophage-synthesis of eicosanoids. Our evidence suggests that degradation of phosphatidylinositol and lyso(bis)phosphatidic acid induced by TPA yields a source of arachidonate which is the preferred substrate for oxidative metabolism by the cyclooxygenase and lipoxygenase pathways.
为了确定用于类花生酸生成的花生四烯酸的来源，用1.0微摩尔12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）或3.8微摩尔钙离子载体A23187刺激兔肺泡巨噬细胞3小时。用TPA刺激后，观察到磷脂酰胆碱、磷脂酰乙醇胺、溶血（双）磷脂酸和磷脂酰丝氨酸/磷脂酰肌醇中[3H]花生四烯酸的丢失。尽管钙离子载体仅刺激花生四烯酸从磷脂酰乙醇胺和磷脂酰胆碱中释放，但事实证明它对巨噬细胞合成类花生酸的刺激作用较弱。我们的证据表明，TPA诱导的磷脂酰肌醇和溶血（双）磷脂酸的降解产生了花生四烯酸的来源，这是环氧化酶和脂氧化酶途径进行氧化代谢的首选底物。
