[某种物质]在蓝莓中对由[某种病原菌]引起的灰霉病的防治应用及作用机制：诱导苯丙烷类途径代谢

Application of for the control of gray mold caused by in blueberry and mechanisms of action: inducing phenylpropanoid pathway metabolism.

作者信息

Du Qianjie, Li Raoyong, Liu Li, Chen Lin, Tang Junrong, Deng Jia, Wang Fang

机构信息

Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming, China.

Forestry College, Southwest Forestry University, Kunming, China.

出版信息

Front Microbiol. 2024 Aug 30;15:1455008. doi: 10.3389/fmicb.2024.1455008. eCollection 2024.

DOI:10.3389/fmicb.2024.1455008

PMID:39282559

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11392732/

Abstract

BACKGROUND

a blueberry gray mold, is one of the main diseases affecting postharvest storage, causing significant losses. Several studies have shown that can prevent the growth of plant pathogens by producing various antibacterial substances, and can induce plant resistance. However, research on the biological management of post-harvest gray mold in blueberries using remains unclear.

METHODS

To better control the postharvest gray mold of blueberry, the effects of KXF6501 fermentation solution (YY) and KXF6501 cell-free supernatant (SQ) on the induction of disease resistance in blueberry fruits were studied using biochemical and transcriptomic analyses.

RESULTS

We found that YY controlled the conidial germination and mycelial growth of , followed by SQ. After 3 d of culture, the lesion diameter and incidence of gray mold in blueberry fruits inoculated with YY and SQ were smaller than those in the control group. Therefore, gray mold in blueberries was effectively controlled during the prevention period, and the control effect of YY was better than that of SQ. Transcription spectrum analysis of blueberry peel tissue showed that the YY- and SQ-induced phenylpropane metabolic pathways had more differentially expressed genes (DEGs) than other biological pathways. In addition, biochemical analyses showed that YY treatment effectively enhanced the activity of enzymes related to the phenylpropane pathway (phenylalanine ammonialyase [PAL], cinnamate 4-hydroxylase [C4H], 4-coumarate CoA ligase [4CL], and polyphenol oxidase [PPO]) and stimulated the synthesis of lignin, total phenols, and flavonoids, followed by SQ. Compared with the control, the YY and SQ treatments reduced the weight loss rate and better maintained the appearance and nutritional quality of the blueberry fruits.

CONCLUSION

Our findings suggest that KXF6501 is potentially useful as a suitable bio-control agent in harvested blueberries.

摘要

背景

蓝莓灰霉病是影响采后贮藏的主要病害之一，会造成重大损失。多项研究表明，[具体物质未提及]可通过产生多种抗菌物质来抑制植物病原菌生长，并能诱导植物抗性。然而，利用[具体物质未提及]对蓝莓采后灰霉病进行生物防治的研究仍不明确。

方法

为更好地控制蓝莓采后灰霉病，采用生化和转录组分析方法，研究了[具体物质未提及]KXF6501发酵液（YY）和KXF6501无细胞上清液（SQ）对蓝莓果实抗病性诱导的影响。

结果

我们发现YY对[病原菌未提及]的分生孢子萌发和菌丝生长的抑制作用优于SQ。培养3天后，接种YY和SQ的蓝莓果实中灰霉病的病斑直径和发病率均小于对照组。因此，在预防期内蓝莓灰霉病得到了有效控制，且YY的防治效果优于SQ。蓝莓果皮组织的转录谱分析表明，与其他生物学途径相比，YY和SQ诱导的苯丙烷代谢途径中差异表达基因（DEG）更多。此外，生化分析表明，YY处理有效提高了苯丙烷途径相关酶（苯丙氨酸解氨酶[PAL]、肉桂酸4-羟化酶[C4H]、4-香豆酸辅酶A连接酶[4CL]和多酚氧化酶[PPO]）的活性，并刺激了木质素、总酚和类黄酮的合成，SQ的效果次之。与对照组相比，YY和SQ处理降低了蓝莓果实的失重率，更好地保持了果实的外观和营养品质。