Chen Jiahui, Zhang Xinyan
Beijing Stomatological Hospital and School of Stomatology, Beijing Institute of Dental Research, Capital Medical University, No. 4 Tiantanxili, Dongcheng District, Beijing 100050, China.
Curr Pharm Des. 2025;31(5):391-412. doi: 10.2174/0113816128328547240827045955.
(PS), the spike from L., is a traditional Chinese medicine that can treat Oral Squamous Cell Carcinoma (OSCC), whereas its molecular mechanisms and effects on the prognosis of patients remain unclear.
Our study aimed to identify the potential anti-OSCC targets of PS and explore its mechanisms and effects on prognosis through network pharmacology, bioinformatics analysis, molecular docking, and cell assays.
Sixty-two potential targets of 11 active anti-OSCC ingredients of PS were identified, with Quercetin, the core ingredient of PS, exhibiting the most significant number of OSCC-related targets. GO analysis indicated that the primary biological processes involved in OSCC treatment by PS were the cellular response to nitrogen compound, response to xenobiotic stimulus, and cellular response to organonitrogen compound. KEGG analysis revealed that pathways in cancer were the top highly enriched signaling pathway in the treatment of OSCC by PS. DisGeNET analysis is mainly about Lip and Oral Cavity Carcinoma. More importantly, 6 of the 62 targets were markedly related to prognosis. Molecular docking revealed high affinities between the key component and the prognosis-related target proteins. Treatment of OSCC cell line SCC-25 with Quercetin could inhibit malignant biological behaviors, such as cell proliferation, colony formation, invasion, and migration, as well as affect the targets related to prognosis and promote autophagy.
Overall, these results suggest that PS plays a significant role in treating and improving the prognosis of OSCC by directly influencing various processes in OSCC.
夏枯草（PS），为唇形科植物夏枯草的果穗，是一种可治疗口腔鳞状细胞癌（OSCC）的传统中药，但其分子机制及对患者预后的影响尚不清楚。
我们的研究旨在通过网络药理学、生物信息学分析、分子对接和细胞实验，确定夏枯草潜在的抗OSCC靶点，并探索其作用机制及对预后的影响。
确定了夏枯草11种活性抗OSCC成分的62个潜在靶点，其中夏枯草的核心成分槲皮素显示出与OSCC相关的靶点数量最多。基因本体（GO）分析表明，夏枯草治疗OSCC所涉及的主要生物学过程是细胞对氮化合物的反应、对外源生物刺激的反应以及细胞对有机氮化合物的反应。京都基因与基因组百科全书（KEGG）分析显示，癌症通路是夏枯草治疗OSCC时高度富集的信号通路。疾病基因网络（DisGeNET）分析主要涉及唇癌和口腔癌。更重要的是，62个靶点中有6个与预后显著相关。分子对接显示关键成分与预后相关靶蛋白之间具有高亲和力。用槲皮素处理OSCC细胞系SCC-25可抑制细胞增殖、集落形成、侵袭和迁移等恶性生物学行为，还可影响与预后相关的靶点并促进自噬。
总体而言，这些结果表明夏枯草通过直接影响OSCC的各种过程，在治疗和改善OSCC预后方面发挥着重要作用。
