Wang Yucong, Xie Zhixin, Li Haitian, Zhang Gongsheng, Liu Rongxu, Han Jianchun, Zhang Lili
College of Food Science, Northeast Agricultural University, Harbin 150030, China.
Heilongjiang Green Food Science Research Institute, Harbin 150030, China.
Food Chem X. 2024 Aug 22;23:101756. doi: 10.1016/j.fochx.2024.101756. eCollection 2024 Oct 30.
Studies have demonstrated the protective effect of milk fat globule membrane (MFGM) on probiotics in harsh environments. However, currently, there are no reports on the encapsulation of probiotics using MFGM. In this study, MFGM and pullulan (PUL) polysaccharide fibers were prepared by electrostatic spinning and used to encapsulate probiotics, with whey protein isolates (WPI)/PUL as the control. The morphology, physical properties, mechanical properties, survival, and stability of the encapsulated GG (LGG) were studied. The results showed that the MFGM/PUL solution had significant effects on pH, viscosity, conductivity, and stability. Electrostatic spinning improved the mechanical properties and encapsulation ability of the polymer formed by MFGM/PUL. LGG encapsulated in MFGM/PUL nanofibers survived rate was higher than WPI/PUL nanofibers in mimic intestinal juice, which could be attributed to the phospholipid content contained in MFGM. These results demonstrate that MFGM is a promising material for probiotic encapsulation, providing an important basis for the potential use of MFGM/PUL nanofibers as a robust encapsulation matrix.
研究表明，乳脂肪球膜（MFGM）在恶劣环境中对益生菌具有保护作用。然而，目前尚无关于使用MFGM封装益生菌的报道。在本研究中，通过静电纺丝制备了MFGM和普鲁兰多糖（PUL）纤维，并用于封装益生菌，以乳清蛋白分离物（WPI）/PUL作为对照。研究了封装的GG（LGG）的形态、物理性质、机械性能、存活率和稳定性。结果表明，MFGM/PUL溶液对pH值、粘度、电导率和稳定性有显著影响。静电纺丝提高了由MFGM/PUL形成的聚合物的机械性能和封装能力。在模拟肠液中，封装在MFGM/PUL纳米纤维中的LGG存活率高于WPI/PUL纳米纤维，这可能归因于MFGM中含有的磷脂含量。这些结果表明，MFGM是一种有前景的益生菌封装材料，为MFGM/PUL纳米纤维作为一种强大的封装基质的潜在应用提供了重要依据。
