Yao Haiyan, Fan Yuan, Emre Emine Sumeyra Turali, Li Na, Ge Min, Wang Jiaolong, Wei Junchao
School of Stomatology, Jiangxi Medical College, Nanchang University, Nanchang 330006, China; School of Chemistry and Chemical Engineering, Nanchang University, Nanchang 330031, China; Jiangxi Province Key Laboratory of Oral Disease, Nanchang 330006, China.
School of Stomatology, Jiangxi Medical College, Nanchang University, Nanchang 330006, China; Jiangxi Province Clinical Research Center for Oral Disease, Nanchang 330006, China.
Int J Biol Macromol. 2024 Sep 17:135739. doi: 10.1016/j.ijbiomac.2024.135739.
Bacterial infections is one of the main factors delaying the wound healing, which has become a serious challenge for healthcare systems. Zinc oxide nanoparticles (ZnO NPs), which show broad-spectrum and excellent antibacterial activity, tend to aggregate easily and therefore hardly penetrate into bacterial biofilms, showing limited anti-biofilm properties. Herein，alginate (ALG) modified ZnO NPs (ZnO@ALG) were prepared via the combination of mussel-inspired method and "thiol-Michael" click reaction, which showed excellent dispersion and biocompatibility. Besides, the interactions between ZnO@ALG and bacteria was much better than that of ZnO NPs, and makes the bacteria produced more reactive oxygen species (ROS) than bare ZnO NPs. The anti-planktonic activity of ZnO@ALG (250 μg/mL) could reach almost 100 %, which was 2-3 times higher than that of bare ZnO NPs. In addition, the ZnO@ALG could significantly accelerate the healing of S. aureus infected wounds, and the wound healing rate of ZnO@ALG group was about 79.2 %, which was significantly higher than that of ZnO NPs (~65.8 %). This study demonstrates that the ZnO@ALG holds a great potential in the anti-planktonic and anti-biofilm fields, and the ALG-modification method can be an effective strategy to enhance the antibacterial properties of nanomaterials.
细菌感染是延缓伤口愈合的主要因素之一，这已成为医疗保健系统面临的严峻挑战。氧化锌纳米颗粒（ZnO NPs）具有广谱且优异的抗菌活性，但容易聚集，因此很难渗透到细菌生物膜中，其抗生物膜性能有限。在此，通过贻贝启发法和“硫醇-迈克尔”点击反应相结合制备了海藻酸钠（ALG）修饰的ZnO NPs（ZnO@ALG），其表现出优异的分散性和生物相容性。此外，ZnO@ALG与细菌之间的相互作用比ZnO NPs更好，并且使细菌产生的活性氧（ROS）比裸露的ZnO NPs更多。ZnO@ALG（250μg/mL）的抗浮游菌活性几乎可达100%，比裸露的ZnO NPs高2至3倍。此外，ZnO@ALG可显著加速金黄色葡萄球菌感染伤口的愈合，ZnO@ALG组的伤口愈合率约为79.2%，明显高于ZnO NPs组（约65.8%）。本研究表明，ZnO@ALG在抗浮游菌和抗生物膜领域具有巨大潜力，且ALG修饰方法可能是增强纳米材料抗菌性能的有效策略。
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