血小板体积的常规测量：光散射技术与孔径阻抗技术的比较

The routine measurement of platelet volume: a comparison of light-scattering and aperture-impedance technologies.

作者信息

Trowbridge E A, Reardon D M, Hutchinson D, Pickering C

出版信息

Clin Phys Physiol Meas. 1985 Aug;6(3):221-38. doi: 10.1088/0143-0815/6/3/003.

Abstract

The effect of dipotassium ethylenediaminetetra-acetic acid (EDTA) on platelet count and mean volume (MPV) was evaluated using two routine measurement systems, a Coulter S Plus (Phase 1) (S+) and a Technicon H6000 (H6000). In normal subjects (n = 29) MPV increased by 17% during 39 h storage in EDTA when measured by the S+. In contrast MPV decreased by 22% when measured by the H6000. MPV differences of up to 40% were observed between the two systems. Concomitant platelet counts, in both systems, changed by less than 4%. A mathematical model of the variation of MPV with storage time was constructed, enabling experimental results to be extrapolated, with accuracy, to time zero (MPV0). The H6000 average MPV0 was significantly larger than the S+ average MPV0. Using the anticoagulant sodium citrate and prostaglandin E1 (NaCitrate-PGE1) there were no significant changes in MPV measured by the S+ during 7 h storage, although a linear decrease in platelet count was observed. A decrease in H6000 MPV was observed whether the blood was stored in EDTA or NaCitrate-PGE1. Methodology, anti-coagulation and storage time all influence MPV. Until these determinants are standardised the clinical value of MPV cannot be assessed.

摘要

使用两种常规测量系统，即库尔特S Plus（第一阶段）（S+）和Technicon H6000（H6000），评估乙二胺四乙酸二钾（EDTA）对血小板计数和平均体积（MPV）的影响。在正常受试者（n = 29）中，当用S+测量时，在EDTA中储存39小时期间MPV增加了17%。相比之下，当用H6000测量时，MPV下降了22%。在这两种系统之间观察到MPV差异高达40%。在这两种系统中，伴随的血小板计数变化均小于4%。构建了MPV随储存时间变化的数学模型，能够将实验结果准确外推至时间零点（MPV0）。H6000的平均MPV0显著大于S+的平均MPV0。使用抗凝剂柠檬酸钠和前列腺素E1（柠檬酸钠 - PGE1）时，在7小时储存期间，S+测量的MPV没有显著变化，尽管观察到血小板计数呈线性下降。无论血液储存在EDTA还是柠檬酸钠 - PGE1中，H6000的MPV均下降。方法学、抗凝和储存时间均会影响MPV。在这些决定因素标准化之前，无法评估MPV的临床价值。