Student Research Committee, Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Science and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Int J Biol Macromol. 2024 Nov;280(Pt 2):135789. doi: 10.1016/j.ijbiomac.2024.135789. Epub 2024 Sep 19.
The need for bioactive-incorporated biodegradable packaging products is growing due to the desire to achieve food goods that have a longer shelf life and enhanced safety. The current study set out to create an edible coating using Malva neglecta seed polysaccharide mucilage (MNSM) containing Lactobacillus brevis TD4-derived postbiotics (PLB), and assess how well the PLB-MNSM edible coating preserved beef slices over a 12-day period of refrigeration. PLB was rich in fatty acids, organic heteropolycyclic compounds, monoterpene and cyclohexanol derivative, prenol lipids, ester compounds, and alpha-CH-containing aldehyde with significant antimicrobial and antioxidant activities. By adding it to the edible coating at 0, 5, 10, and 15 % v/v, it successfully prevented the proliferation of microbial agents (total viable count, psychrotrophic count, Staphylococcus aureus, Escherichia coli, total coliform bacteria count, and fungi) as well as the oxidation of lipids (thiobarbituric and peroxide values) in beef samples. The samples' pH value, hardness, and moisture content were all more successfully sustained when PLB preparation was applied to the coating solution (P < 0.05). The edible coating consisting of PLB effectively maintained the meat color (L*, a*, b*) and sensory properties. Additionally, the bioactive edible coating comprised of MNSM and PLB, specifically MNSM-15 % PLB, significantly prevented the quality deterioration of beef samples and prolonged the shelf-life of the meat to over 12 days. The outcomes indicated that the MNSM-PLB edible coating has the capacity to be utilized as an antibacterial and antioxidant-rich packing material, hence enhancing the shelf life of meat-based goods.
由于人们希望获得保质期更长且安全性更高的食品,因此对含有生物活性物质的可生物降解包装产品的需求不断增长。本研究旨在利用含有短乳杆菌 TD4 后生元(PLB)的锦葵科植物多糖胶(MNSM)制备可食用涂层,并评估 PLB-MNSM 可食用涂层在冷藏 12 天期间对牛肉切片的保鲜效果。PLB 富含脂肪酸、有机杂环化合物、单萜和环己醇衍生物、prenol 脂质、酯类化合物和含α-CH 的醛,具有显著的抗菌和抗氧化活性。通过将其以 0、5、10 和 15% v/v 的比例添加到可食用涂层中,成功抑制了微生物制剂(总活菌数、嗜冷菌计数、金黄色葡萄球菌、大肠杆菌、总大肠菌群计数和真菌)的增殖以及牛肉样品中脂质(硫代巴比妥酸和过氧化物值)的氧化。当将 PLB 制剂应用于涂层溶液时,样品的 pH 值、硬度和水分含量均得到更好的维持(P<0.05)。由 PLB 组成的可食用涂层能有效保持肉色(L*、a*、b*)和感官特性。此外,由 MNSM 和 PLB 组成的生物活性可食用涂层,特别是 MNSM-15% PLB,能显著防止牛肉样品质量恶化,使肉的保质期延长至 12 天以上。研究结果表明,MNSM-PLB 可食用涂层具有作为一种富含抗菌和抗氧化剂的包装材料的潜力,从而延长肉类制品的保质期。