Overexpression of Nicotiana tabacum PIP1;3 enhances root aeration and oxygen metabolism in canola (Brassica napus) plants exposed to root hypoxia.

High mortality and reduced growth due to root hypoxia are commonly observed in plants impacted by flooding or soil compaction. Since earlier research suggested that Nicotiana tabacum PIP1;3 may facilitate cell-to-cell oxygen transport, we overexpressed NtPIP1;3 in canola (Brassica napus) and studied the effects on growth, physiological parameters, root oxygen concentrations, and energy metabolism in plants subjected to waterlogging. Compared with wild-type plants (WT), the waterlogged plants overexpressing NtPIP1;3 (OE) maintained higher dry biomass, gas exchange, root hydraulic conductivity, root oxygen concentrations, leaf water potentials, root respiration rates, and root ATP concentrations. Metabolic profiling revealed that overexpressing plants responded to root hypoxia by altering the glycolysis, pyruvate metabolism, and TCA cycle in roots. Moreover, the differences in expression patterns of RAP2.12, RAP2.2, PCO1, and PCO2 in WT and OE canola plants exposed to root hypoxia point to increased oxygen supply to OE roots, which was confirmed by direct measurements of root O concentrations. Our results demonstrate that the overexpression of NtPIP1;3 affected plant responses to hypoxia by enhancing their aerobic metabolism and strengthened the notion that some of the plant aquaporins may facilitate oxygen transport.