School of Food Science and Engineering, Hainan University, Haikou 570228, PR China.
School of Food Science and Engineering, Hainan University, Haikou 570228, PR China; Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Hainan University, Haikou 570228, PR China.
Food Chem. 2025 Jan 15;463(Pt 2):141329. doi: 10.1016/j.foodchem.2024.141329. Epub 2024 Sep 17.
Aquilaria sinensis leaves have long been consumed as a popular replacement tea for lowering postprandial blood glucose levels, but their specific functional components remain unclear. In this study, Aquilaria sinensis leaf-tea 70 % ethanol extract (ALTE) exhibited excellent anti-α-glucosidase activity (IC = 6.93 ± 1.91 μg/mL) and promoted glucose consumption ability in 3 T3-L1 preadipocyte cells. Subsequently phenolic compositions of ALTE were identified for the first time. After that, five potential α-glucosidase inhibitors (α-GIs) including cynaroside-3,5-diglucose, malvidin 3-glucose, epicatechin, epigallocatechin gallate, and dihydromyricetin in ALTE were screened using a targeted bio-affinity ultrafiltration-HPLC/MS method. Moreover, these five α-GIs all showed good anti-α-glucosidase effects and glucose consumption-promoting ability. Furthermore, the binding properties and inhibition mechanisms of five α-GIs to α-glucosidase were further analyzed via enzyme inhibition kinetics, molecular docking, and molecular dynamics simulation. This study confirms that Aquilaria sinensis leaf-tea is effective in preventing post-hyperglycemia in vitro models, suggesting potential for future research in human trials.
白木香叶长期以来一直被用作降低餐后血糖水平的流行代茶，但它们的特定功能成分仍不清楚。在这项研究中，白木香叶茶 70%乙醇提取物（ALTE）表现出优异的抗α-葡萄糖苷酶活性（IC=6.93±1.91μg/mL），并能促进 3T3-L1 前体脂肪细胞的葡萄糖消耗能力。随后首次鉴定了 ALTE 的酚类成分。之后，采用靶向生物亲和超滤 HPLC/MS 方法筛选了 ALTE 中的 5 种潜在的α-葡萄糖苷酶抑制剂（α-GIs），包括梓醇-3,5-二葡萄糖、矢车菊素-3-葡萄糖、表儿茶素、没食子酸表儿茶素酯和二氢杨梅素。此外，这 5 种 α-GIs 均表现出良好的抗α-葡萄糖苷酶作用和促进葡萄糖消耗能力。此外，还通过酶抑制动力学、分子对接和分子动力学模拟进一步分析了 5 种 α-GIs 与α-葡萄糖苷酶的结合特性和抑制机制。这项研究证实，白木香叶茶在体外模型中有效预防高血糖后状态，提示在临床试验中进行进一步研究的潜力。
